Date published: 2025-9-9

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Anticorps Id1 (F-10): sc-365654

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Fiches techniques
  • L'Anticorps Id1 (F-10) est un monoclonal de souris IgG1 κ, cité dans 2 publications, fourni en 200 µg/ml
  • dirigé contre les acides aminés 1-154 correspondant à la séquence totale de Id1 d'origine human
  • recommandé pour la détection de Id1 d'origine human par WB, IP, IF et ELISA
  • Réactifs TransCruz pour une application en ChIP (sc-365654 X, 200 µg/0.1 ml)
  • Voir Id1 (B-8): sc-133104 pour d'autres anticorps conjugués Id1, dont AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 et 790.
  • m-IgG Fc BP-HRP, 1 BP-HRP">m-IgG1 BP-HRP et m-IgGκ BP-HRP sont les réactifs de détection secondaire préférés pour l'anticorps Id1 (F-10) pour les applications WB. Ces réactifs sont désormais proposés en lots avec l'anticorps Id1 (F-10)(voir les informations de commande ci-dessous).

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

Members of the Id family of basic helix-loop-helix (bHLH) proteins include Id1, Id2, Id3 and Id4. They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA. The Id-type proteins thus appear to negatively regulate DNA binding of bHLH proteins. Since Id1 inhibits DNA binding of E12 and Myo D, it apparently functions to inhibit muscle-specific gene expression. Under conditions that facilitate muscle cell differentiation, the Id protein levels fall, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which in turn activate myogenic differentiation. It has been shown that expression of each of the Id proteins is strongly dependent on growth factor activation and that reduction of Id mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps Id1 (F-10)

sc-365654
200 µg/ml
$316.00

Id1 (F-10): m-IgG Fc BP-HRP Kit

sc-537821
200 µg Ab; 10 µg BP
$354.00

Id1 (F-10): m-IgGκ BP-HRP Kit

sc-535233
200 µg Ab; 40 µg BP
$354.00

Id1 (F-10): m-IgG1 BP-HRP Kit

sc-545376
200 µg Ab; 20 µg BP
$354.00

Anticorps Id1 (F-10) X

sc-365654 X
200 µg/0.1 ml
$316.00

What is the recommended fixation method for immunofluorescence staining with Id1 (F-10): sc-365654 monoclonal antibody?

Posée par: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Répondue par: Technical Support
Date de publication: 2017-02-24
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Rated 5 de 5 de par Produced great immunofluorescence nuclearProduced great immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date de publication: 2015-03-27
Rated 5 de 5 de par Great Western Blot data of Id1 expression in nonGreat Western Blot data of Id1 expression in non-transfected and human Id1 transfected 293T whole cell lysates. -SCBT QC
Date de publication: 2014-12-12
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Anticorps Id1 (F-10) est évalué 5.0 de 5 de 2.
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