Date published: 2025-9-5

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Anticorps Histone H3 (1G1): sc-517576

4.7(10)
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Fiches techniques
  • L'anticorps Histone H3 1G1 est un monoclonal IgG de souris, cité dans 87 publications, fourni en 100 µg/ml
  • dirigée contre des fragments de protéines Histone H3 recombinantes d'origine human.
  • recommandé pour la détection de Histone H3 d'origine mouse, rat et human par WB et IF
  • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de Histone H3 (1G1): sc-517576.
  • m-IgG Fc BP-HRP et 1 BP-HRP">m-IgG1 BP-HRP sont les réactifs de détection secondaire préférés pour Histone H3 Antibody (1G1) for WB applications. Ces réactifs sont désormais proposés en lots avec Histone H3 Antibody (1G1)(voir les informations de commande ci-dessous).

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

L'anticorps Histone H3 (1G1) est un anticorps IgG monoclonal de souris qui détecte l'Histone H3 dans des échantillons de souris, de rats et d'humains par western blotting (WB) et immunofluorescence (IF). L'anticorps anti-Histone H3 (1G1) est disponible sous forme non conjuguée. L'histone H3 joue un rôle crucial dans l'organisation structurelle de la chromatine, essentielle pour l'empaquetage de l'ADN dans le noyau. L'histone H3 forme une partie centrale de la structure octamérique du nucléosome qui enveloppe l'ADN, régulant l'expression des gènes et la réplication de l'ADN. L'histone H3 maintient la stabilité du génome et facilite les processus de transcription et de réparation. L'histone H3 subit diverses modifications post-traductionnelles, notamment la méthylation et l'acétylation, qui influencent les interactions entre l'ADN et les protéines, ainsi que la structure et la fonction de la chromatine. Ces modifications régulent l'expression des gènes et ont un impact sur la différenciation cellulaire et la réponse environnementale. Les capacités de détection de l'anticorps anti-histone H3 (1G1) rendent ce réactif précieux pour l'étude de la dynamique de la chromatine et de la régulation épigénétique dans divers contextes biologiques.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps Histone H3 (1G1)

sc-517576
100 µg/ml
$316.00

Histone H3 (1G1): m-IgG Fc BP-HRP Kit

sc-541115
100 µg Ab; 10 µg BP
$354.00

Histone H3 (1G1): m-IgG1 BP-HRP Kit

sc-542591
100 µg Ab; 20 µg BP
$354.00

I performed ICC with this antibody in mitotic cells, but I couldn't detect histone H3 signal co-localized with the DAPI signal. Could you help me solve this issue?

Posée par: KWJ94
Thank you for your product review. We are sorry to hear that this antibody did not perform for you as expected. There are many possible causes for no signal. Please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website, and we would be happy to help you figure this out. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase and will do our best to help you achieve the best results possible!
Répondue par: Jiajun Z
Date de publication: 2024-09-29

Hi can we please get a sample of sc-517576? Do you have that available? We just purchased sc-390935 with Texas A&M University Biology Department, Dr. Deb Bell-Pedersen lab.

Posée par: Johnny
Thank you for contacting Santa Cruz Biotechnology. To request a sample please email scbt@scbt.com
Répondue par: BlakeJ
Date de publication: 2024-06-18

Just received a free sample, which dilution would you recommend for nuclear extracts WB?

Posée par: IngOlmos
Thank you for your question. Dilution recommendations can be found in the product's datasheet, which is available right here on the product page. For western blots we recommend a starting dilution of 1:200, and a dilution range between 1:100-1:1000. When working with nuclear extracts, an amount of protein between 10–20 µg is appropriate.
Répondue par: Tech Support Europe
Date de publication: 2023-05-15

could it be possible to buy directly bound to alexa488 or 594 please

Posée par: antonio blesa
Thank you for your question. If you are interested in this antibody directly conjugated with AF488 or AF594, please contact our our Technical Service. We should be able to provide this format as special order.
Répondue par: SCBT Heidelberg Support
Date de publication: 2018-12-31
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Rated 4 de 5 de par Good work!!This antibody gave a strong and clean signal when we used it at a 1:100 diluition with 80μg of protein samples.
Date de publication: 2023-02-17
Rated 5 de 5 de par H3 Ab for Western BlottingAsked for a sample before purchasing. Worked straight away on CRC cell lines HCT116 and DLD1 (drug treated and untreated) when loading 20 ug of whole protein lysates and using 1:500 dilution in 5% BSA/TBST. Would definitely recommend.
Date de publication: 2023-01-24
Rated 5 de 5 de par western blotI used this antibody for my mouse primary neuron cells western blot. I got very nice and clean bolt!
Date de publication: 2022-08-24
Rated 5 de 5 de par good and strongThe antibody was tested on whole lysate from liver cancer cell lines (JHH6 andHuh7) and immortalized hepatocytes (IHH). Antibody: sensitive and specific. Too strong signal with the dilution you recommend. Conditions need to be better set. Apparent MW: about 15kDa. Expected MW: 15kDa. • 40ug/lane cell lysate • Transfer 1h30 100V, PVDF (0.2 µm) • Blocking milk 4% in 0.1% Tween -PBS 1h RT • Anti-HISTONE H3 (1G1)(sc-517576) 1:200 O/N 4°C in blocking solution • Anti-mouse HRP 1:1000 1h RT in blocking solution • Developed with ECL Plus
Date de publication: 2022-06-09
Rated 5 de 5 de par Very good!In MB231 cells, a clear band appeared after WB analysis 1: 2000 dilution.
Date de publication: 2022-03-18
Rated 4 de 5 de par Useful for my purposesI asked it as free trial and i worked well enough, so I think I can use it for my purposes.
Date de publication: 2020-12-23
Rated 5 de 5 de par Great for IFBought this antibody recently for the purpose of immunofluorescence on human neuroblastoma cell lines. It worked very well.
Date de publication: 2019-09-03
Rated 5 de 5 de par Works very well with 1:5000 dilutionWorks very well with 1:5000 dilution, and I hope some more conjugation products can be developed with this antibody.
Date de publication: 2019-02-05
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Anticorps Histone H3 (1G1) est évalué 4.7 de 5 de 10.
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