Date published: 2025-9-8

00800 4573 8000

SCBT Portrait Logo
Seach Input

Anticorps Hec1 (1): sc-135934

5.0(3)
Écrire une critiquePoser une question

Fiches techniques
  • L'anticorps Hec1 1 est un monoclonal IgG1 de souris, cité dans 4 publications, fourni en 50 µg/500 µl
  • dirigé contre les acides aminés 495-608 de Hec1 d'origine human
  • recommandé pour la détection de Hec1 d'origine mouse, rat, human et canine par WB, IP et IF
  • Nous testons actuellement encore nos anticorps secondaires pour trouver la meilleure protéine de liaison pour cet anticorps primaire Hec1 (1). Veuillez nous contacter si vous avez des questions concernant les réactifs de détection secondaire appropriés.

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

Highly expressed in cancer (Hec1) is a coiled-coil-enriched protein expressed abundantly in the S and M phases of rapidly dividing cells where it localizes to the kinetochores. Hec1 is involved in spindle checkpoint signaling. Hec1 is not expressed in terminal differentiated cells. Hec1 is expressed in tissues with high mitotic rates including testis, spleen and thymus. Hec1 is also found in the late S to M phases of bladder carcinoma cells. In dividing cells, Hec1 is required for the recruitment of Mps1 kinase and MAD1/MAD2 complexes to the kinetochores. The phosphorylation of Hec1 on Serine 165 by Nek2 is essential for faithful chromosome segregation. The binding of retinoblastoma protein to Hec1 also increases the fidelity of chromosomal segregation.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps Hec1 (1)

sc-135934
50 µg/500 µl
$316.00

I am using Hec1 (1): sc-135934 for immunofluorescence, what dilution of this antibody should I use?

Posée par: AbPolly
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Répondue par: Technical Support
Date de publication: 2017-03-24
  • y_2025, m_8, d_29, h_8CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_fr_FR, sid_135934, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 111ms
  • QUESTIONS, PRODUCT
Rated 5 de 5 de par gave a single bandRan cell lysate on a 10 percent gel and transfered to PVDF using 10% Methanol Transfer buffer in a semi dry apparatus. Antibody gave a single band at approximately the expected molecular weight. Hec1 knockdown by siRNA was reflected in the band intensity by western.
Date de publication: 2017-02-15
Rated 5 de 5 de par Excellent immunofluorescence cytoplasmicExcellent immunofluorescence cytoplasmic staining in BC3H1 cells. -SCBT QC
Date de publication: 2015-02-25
  • y_2025, m_8, d_29, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_2
  • loc_fr_FR, sid_135934, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 13ms
  • REVIEWS, PRODUCT
Anticorps Hec1 (1) est évalué 5.0 de 5 de 3.
  • y_2025, m_8, d_29, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_2
  • loc_fr_FR, sid_135934, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 104ms
  • REVIEWS, PRODUCT