Glutathione Peroxidase 4/GPX4 Antibody (E-12): sc-166570

GPx-4 Antibody (E-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects GPx-4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-GPx-4 antibody (E-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GPx-4 plays a crucial role in cellular defense against oxidative stress, primarily by reducing lipid hydroperoxides to their corresponding alcohols and converting free hydrogen peroxide into water, thereby preventing lipid peroxidation of cell membranes. GPx-4 is unique among glutathione peroxidases as GPx-4 contains selenocysteine at its active site, which is essential for enzymatic function and is encoded by the stop codon TGA. This post-translational modification allows GPx-4 to maintain antioxidant activity, which is particularly important in protecting cells from oxidative damage that can lead to various diseases, including neurodegenerative disorders and cancer. GPx-4 forms high molecular weight oligomers, which may enhance stability and functional capacity in cellular environments. The diverse roles of GPx-4 in different tissues underscore significance in maintaining cellular homeostasis and responding to oxidative challenges.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Glutathione Peroxidase 4/GPX4 Antibody (E-12) References:

1. Selenium Utilization by GPX4 Is Required to Prevent Hydroperoxide-Induced Ferroptosis.  |  Ingold, I., et al. 2018. Cell. 172: 409-422.e21. PMID: 29290465
2. Role of GPX4 in ferroptosis and its pharmacological implication.  |  Seibt, TM., et al. 2019. Free Radic Biol Med. 133: 144-152. PMID: 30219704
3. Legumain promotes tubular ferroptosis by facilitating chaperone-mediated autophagy of GPX4 in AKI.  |  Chen, C., et al. 2021. Cell Death Dis. 12: 65. PMID: 33431801
4. Wnt/beta-catenin signaling confers ferroptosis resistance by targeting GPX4 in gastric cancer.  |  Wang, Y., et al. 2022. Cell Death Differ. 29: 2190-2202. PMID: 35534546
5. Thiostrepton induces ferroptosis in pancreatic cancer cells through STAT3/GPX4 signalling.  |  Zhang, W., et al. 2022. Cell Death Dis. 13: 630. PMID: 35859150
6. Baicalin induces ferroptosis in osteosarcomas through a novel Nrf2/xCT/GPX4 regulatory axis.  |  Wen, RJ., et al. 2023. Phytomedicine. 116: 154881. PMID: 37209607
7. HDAC3 aberration-incurred GPX4 suppression drives renal ferroptosis and AKI-CKD progression.  |  Zhang, L., et al. 2023. Redox Biol. 68: 102939. PMID: 37890360
8. SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation.  |  Zhang, W., et al. 2023. Mol Cell. 83: 4352-4369.e8. PMID: 38016474
9. m6A and m5C modification of GPX4 facilitates anticancer immunity via STING activation.  |  Chen, B., et al. 2023. Cell Death Dis. 14: 809. PMID: 38065948
10. Autophagy of OTUD5 destabilizes GPX4 to confer ferroptosis-dependent kidney injury.  |  Chu, LK., et al. 2023. Nat Commun. 14: 8393. PMID: 38110369

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Glutathione Peroxidase 4/GPX4 Antibody (E-12) has 87 citations in a variety of scientific publications.

Primary antibodies like Anti-Glutathione Peroxidase 4/GPX4 Antibody (E-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Glutathione Peroxidase 4/GPX4 Antibody (E-12) Specifications

Alternate Product Name: Glutathione Peroxidase 4

Product Clone Name: E-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 108-197

Epitope Target: GPx-4

Epitope Species: human

Product Reactivity/Detection: GPx-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 87 citations in various scientific publications

Available Conjugates/Forms for Glutathione Peroxidase 4/GPX4 Antibody (E-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Glutathione Peroxidase 4/GPX4 antibody is Glutathione Peroxidase 4/GPX4 Antibody (B-12): sc-166120.