Glucose Transporter Glut4 Antibody (IF8): sc-53566

Glut4 Antibody (IF8) is a mouse monoclonal IgG1 kappa light chain antibody raised against partially purified vesicles containing Glut4 from rat adipocytes, with reactivity in mouse, rat, and human species. Anti-Glut4 antibody (IF8) is validated for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Glut4, also known as Glucose Transporter Type 4 or SLC2A4, is a 12-pass transmembrane protein responsible for insulin-regulated glucose uptake in adipose and muscle tissues. Glut4 translocates from intracellular storage vesicles to the plasma membrane in response to insulin, facilitating glucose entry into cells. Unlike Glut1, which is constitutively present at the plasma membrane, Glut4′s dynamic regulation is essential for adapting to varying metabolic demands. This function maintains energy balance, and disruptions in Glut4 expression or trafficking are linked to metabolic disorders like obesity and type 2 diabetes. Studies in Glut4 knockout mice demonstrate essential roles in growth, glucose metabolism, fat storage, and overall longevity. Anti-Glut4 antibody (IF8) serves as a valuable tool for exploring mechanisms of glucose regulation, insulin signaling, and related metabolic pathways in health and disease contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Glucose Transporter Glut4 Antibody (IF8) References:

1. Molecular regulation of GLUT-4 targeting in 3T3-L1 adipocytes.  |  Marsh, BJ., et al. 1995. J Cell Biol. 130: 1081-91. PMID: 7544796
2. Regulation of glucose transporters in cultured rat adipocytes: synergistic effect of insulin and dexamethasone on GLUT4 gene expression through promoter activation.  |  Hajduch, E., et al. 1995. Endocrinology. 136: 4782-9. PMID: 7588207
3. Cardiac and adipose tissue abnormalities but not diabetes in mice deficient in GLUT4.  |  Katz, EB., et al. 1995. Nature. 377: 151-5. PMID: 7675081
4. Insulin-sensitive targeting of the GLUT4 glucose transporter in L6 myoblasts is conferred by its COOH-terminal cytoplasmic tail.  |  Haney, PM., et al. 1995. J Cell Biol. 129: 641-58. PMID: 7730401
5. Expression and compartmentalization of caveolin in adipose cells: coordinate regulation with and structural segregation from GLUT4.  |  Kandror, KV., et al. 1995. J Cell Biol. 129: 999-1006. PMID: 7744970
6. Hypothalamic GLUT 4 expression: a glucose- and insulin-sensing mechanism?  |  Livingstone, C., et al. 1995. Mol Cell Endocrinol. 107: 67-70. PMID: 7796936
7. Evidence that facilitative glucose transporters may fold as beta-barrels.  |  Fischbarg, J., et al. 1993. Proc Natl Acad Sci U S A. 90: 11658-62. PMID: 8265604

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Glucose Transporter Glut4 Antibody (IF8) has 151 citations in a variety of scientific publications.

Primary antibodies like Anti-Glucose Transporter Glut4 Antibody (IF8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Glucose Transporter Glut4 Antibody (IF8) Specifications

Alternate Product Name: Glut4

Product Clone Name: IF8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Glut4

Epitope Species: rat

Product Reactivity/Detection: Glut4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 151 citations in various scientific publications

Available Conjugates/Forms for Glucose Transporter Glut4 Antibody (IF8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin