Gastric Lipase Antibody (B-2): sc-398343

Gastric Lipase Antibody (B-2) is a mouse monoclonal IgG3 antibody that detects Gastric Lipase in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Gastric Lipase is a 379-amino acid protein that plays a vital role in the digestion of dietary triglycerides, particularly in individuals with pancreatic lipase deficiencies, making it essential for proper nutrient absorption. This enzyme is secreted by the fundic mucosa of the stomach and operates optimally under acidic pH conditions, where it hydrolyzes the ester bonds of triglycerides, facilitating their breakdown into free fatty acids and glycerol. The ability of Gastric Lipase to function effectively in the harsh acidic environment of the stomach underscores its importance in lipid metabolism and highlights its potential implications in digestive disorders. Anti-Gastric Lipase antibody (B-2) is an invaluable tool for researchers studying lipid metabolism and gastrointestinal health, providing insights into the physiological and pathological roles of this critical enzyme.

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Gastric Lipase Antibody (B-2) References:

1. Crystal structure of human gastric lipase and model of lysosomal acid lipase, two lipolytic enzymes of medical interest.  |  Roussel, A., et al. 1999. J Biol Chem. 274: 16995-7002. PMID: 10358049
2. Inhibition of human gastric lipase by intraduodenal fat involves glucagon-like peptide-1 and cholecystokinin.  |  Wøjdemann, M., et al. 1999. Regul Pept. 80: 101-6. PMID: 10425652
3. Cephalic phase of lipolysis is impaired in pancreatic insufficiency: role of gastric lipase.  |  Wøjdemann, M., et al. 2000. Scand J Gastroenterol. 35: 204-11. PMID: 10720121
4. Mechanisms of inhibition of triacylglycerol hydrolysis by human gastric lipase.  |  Pafumi, Y., et al. 2002. J Biol Chem. 277: 28070-9. PMID: 11940604
5. Gastric function in children with cystic fibrosis: effect of diet on gastric lipase levels and fat digestion.  |  Armand, M., et al. 2004. Pediatr Res. 55: 457-65. PMID: 14681491
6. Expression of active recombinant human gastric lipase in Nicotiana benthamiana using the CPMV-HT transient expression system.  |  Vardakou, M., et al. 2012. Protein Expr Purif. 81: 69-74. PMID: 21945702
7. The complete digestion of human milk triacylglycerol in vitro requires gastric lipase, pancreatic colipase-dependent lipase, and bile salt-stimulated lipase.  |  Bernbäck, S., et al. 1990. J Clin Invest. 85: 1221-6. PMID: 2318975
8. Pancreatic lipase-related protein 2 digests fats in human milk and formula in concert with gastric lipase and carboxyl ester lipase.  |  Johnson, K., et al. 2013. Pediatr Res. 74: 127-32. PMID: 23732775
9. Dietary fat modulates gastric lipase activity in healthy humans.  |  Armand, M., et al. 1995. Am J Clin Nutr. 62: 74-80. PMID: 7598069
10. Human lysosomal acid lipase/cholesteryl ester hydrolase and human gastric lipase: identification of the catalytically active serine, aspartic acid, and histidine residues.  |  Lohse, P., et al. 1997. J Lipid Res. 38: 892-903. PMID: 9186907

The preferred LAL antibody is LAL Antibody (9G7F12): sc-58374.