GADD 45β Antibody (G-11): sc-377311

GADD 45β Antibody (G-11) is a mouse monoclonal IgM antibody that detects GADD 45β in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-GADD 45β antibody (G-11) is available in a non-conjugated form, allowing for versatile experimental use. GADD 45β plays a crucial role in cellular response to DNA damage, particularly in regulating cell cycle checkpoints. GADD 45β is primarily located in the nucleus, where GADD 45β interacts with various proteins involved in DNA repair and cell cycle regulation. Notably, GADD 45β binds to cyclin-dependent kinases (Cdks) and proliferating cell nuclear antigen (PCNA), which are essential for DNA replication and repair processes. This interaction helps coordinate cellular response to DNA damage, ensuring repair mechanisms are activated before cells proceed to DNA synthesis and mitosis. GADD 45β stimulates DNA excision repair in vitro and inhibits cell entry into S phase, underscoring GADD 45β′s importance in maintaining genomic stability. GADD 45β and homologs, GADD 45α and GADD 45γ, are induced by various environmental stresses, linking these proteins to cellular stress responses and tumor suppression pathways.

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GADD 45β Antibody (G-11) References:

1. Wild-type p53 is a cell cycle checkpoint determinant following irradiation.  |  Kuerbitz, SJ., et al. 1992. Proc Natl Acad Sci U S A. 89: 7491-5. PMID: 1323840
2. Creative blocks: cell-cycle checkpoints and feedback controls.  |  Murray, AW. 1992. Nature. 359: 599-604. PMID: 1406993
3. A mammalian cell cycle checkpoint pathway utilizing p53 and GADD45 is defective in ataxia-telangiectasia.  |  Kastan, MB., et al. 1992. Cell. 71: 587-97. PMID: 1423616
4. GADD45β Loss Ablates Innate Immunosuppression in Cancer.  |  Verzella, D., et al. 2018. Cancer Res. 78: 1275-1292. PMID: 29279355
5. Reprogramming immunosuppressive tumour-associated dendritic cells with GADD45β inhibitors.  |  Rajpoot, S., et al. 2020. Clin Med (Lond). 20: s116. PMID: 32409418
6. Hepatic Gadd45β promotes hyperglycemia and glucose intolerance through DNA demethylation of PGC-1α.  |  Wu, L., et al. 2021. J Exp Med. 218: PMID: 33688917
7. Gadd45β is critical for regulation of type I interferon signaling by facilitating G3BP-mediated stress granule formation.  |  Chathuranga, WAG., et al. 2023. Cell Rep. 42: 113358. PMID: 37917584
8. Large-scale phosphoproteomics reveals activation of the MAPK/GADD45β/P38 axis and cell cycle inhibition in response to BMP9 and BMP10 stimulation in endothelial cells.  |  Al Tarrass, M., et al. 2024. Cell Commun Signal. 22: 158. PMID: 38439036
9. Contradictory Role of Gadd45β in Liver Diseases.  |  Wu, C., et al. 2024. J Cell Mol Med. 28: e70267. PMID: 39653679
10. New link found between p53 and DNA repair.  |  Marx, J. 1994. Science. 266: 1321-2. PMID: 7973719
11. Interaction of the p53-regulated protein Gadd45 with proliferating cell nuclear antigen.  |  Smith, ML., et al. 1994. Science. 266: 1376-80. PMID: 7973727
12. A family of stress-inducible GADD45-like proteins mediate activation of the stress-responsive MTK1/MEKK4 MAPKKK.  |  Takekawa, M. and Saito, H. 1998. Cell. 95: 521-30. PMID: 9827804

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GADD 45β Antibody (G-11) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-GADD 45β Antibody (G-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GADD 45β Antibody (G-11) Specifications

Alternate Product Name: GADD 45 beta

Product Clone Name: G-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 133-160

Epitope Target: GADD 45β

Epitope Species: human

Product Reactivity/Detection: GADD 45β

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for GADD 45β Antibody (G-11): P: Blocking Peptide