Anticorps Fibrillarin (G-8) recommandé pour la détection de Fibrillarin d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA

Anticorps Fibrillarin (G-8): sc-374022

Anticorps Fibrillarin (G-8) est évalué 4.7 de 5 de 7.
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Fiches techniques

    • Anti-L'anticorps Fibrillarin G-8 est un monoclonal IgG1 κ L'Anticorps Fibrillarin, cité dans 8 publications, fourni en 200 µg/ml
    • spécifique d'un épitope situé entre les acides aminés 99-127 au niveau d'une région interne de Fibrillarin d'origine human
    • Anti-L'Anticorps Fibrillarin (G-8) est recommandé pour la détection de Fibrillarin d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA; également réactif avec d'autres espèces, dont et canine, bovine and porcine
    • Anti-L'Anticorps Fibrillarin (G-8) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de Fibrillarin (G-8): sc-374022.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Fibrillarin Antibody (G-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with Fibrillarin Antibody (G-8) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

I was wondering whether you have any information regarding room temp incubation of this antibody (sc-374022) for IF on cells? I'm planning to use this for a drug screen and the system is not amenable to o/n incubation at 4C. Thanks.

Posée par: Ceryl
Thank you for your question. Yes, we do recommend incubating with this antibody for 60 minutes at room temperature. Please refer to our immunofluorescence protocol for more detailed information.
Répondue par: Technical Support
Date de publication: 2019-03-03

What application is the blocking peptide sc-374022 P appropriate for?

Posée par: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Répondue par: Technical Support
Date de publication: 2017-02-15
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Rated 3 de 5 de par Works ok for IF. akI got a sample of this antibody. Clear but weak IF with 1:50 dilution.
Date de publication: 2017-07-27
Rated 5 de 5 de par great features! Works well for immunofluorescence and Wester blotting in human HT1080 cancer cells and MEF cells.
Date de publication: 2017-07-10
Rated 5 de 5 de par an excellent antibody for both WB and IF This antibody works very well for WB (40 ug U251 Lysate) and IF (formaldehyde-fixed HeLa cells) at 1:500 dilution.
Date de publication: 2017-07-05
Rated 5 de 5 de par Great for IF and co-localization experiments I used a sample of this antibody for co-localization studies in mouse ES cells. The nucleolar staining was very specific and significant. generally pleased with outcome. Will recommend it!
Date de publication: 2017-06-05
Rated 5 de 5 de par Broad reactivity. This antibody is capable of detecting Fibrillarin multiple different species. Both staining and blotting assays provide clear and strong signal. This antibody has been used for publishing.
Date de publication: 2017-01-27
Rated 5 de 5 de par Strong Strong, clear bands present in Western blot analysis of Fibrillarin expression in HeLa and NIH/3T3 whole cell lysates. -SCBT QC
Date de publication: 2015-04-18
Rated 5 de 5 de par Superb immunoperoxidase nucleolar and nuclear Superb immunoperoxidase nucleolar and nuclear staining in formalin fixed, paraffin-embedded human breast tissue. -SCBT QC
Date de publication: 2015-04-10
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