Estrogen Receptor beta Double Nickase Plasmid (h): sc-400213-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Estrogen Receptor beta Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Estrogen Receptor beta Double Nickase Plasmid (h) and Estrogen Receptor beta Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ESR2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Estrogen Receptor beta Antibody (B-3): sc-373853
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Estrogen Receptor beta Double Nickase Plasmid (h) | sc-400213-NIC | 20 µg | $410.00 | |||
Estrogen Receptor beta Double Nickase Plasmid (h2) | sc-400213-NIC-2 | 20 µg | $410.00 |
Human ESR2 encodes estrogen receptor beta (ERβ), a ligand-activated nuclear receptor that regulates transcriptional programs controlling cell proliferation, differentiation, apoptosis, and immune modulation in response to estrogens and related ligands. ERβ functions through classical estrogen response elements and tethered interactions with transcription factors, integrating signaling across MAPK/PI3K-AKT pathways and chromatin remodeling to shape context-dependent gene expression. In many tissues, ERβ counterbalances ERα-driven transcription and contributes to epithelial homeostasis, mitochondrial function, and inflammatory signaling. Dysregulated ESR2 activity or altered ERβ expression has been associated with hormone-responsive cancers, reproductive and metabolic phenotypes, and neuroinflammatory processes, making it a key target for mechanistic studies of estrogen signaling.
Estrogen Receptor beta Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ESR2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ESR2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ESR2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ESR2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.