DNA Ligase I Antibody (F-5): sc-390217

DNA Ligase I Antibody (F-5) is a mouse monoclonal IgG2b antibody that detects DNA Ligase I (LIGI) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DNA Ligase I is crucial for maintaining genome integrity, playing a vital role in DNA replication and repair by catalyzing phosphodiester bond formation between adjacent 5′ phosphoryl and 3′ hydroxy termini at single breaks in duplex DNA molecules. DNA Ligase I function is particularly important during Okazaki fragment joining on the lagging strand during DNA replication, ensuring newly synthesized DNA remains continuous and functional. DNA Ligase I also seals nicks during excision repair, essential for correcting DNA damage that can lead to mutations and genomic instability. Elevated DNA Ligase I levels have been observed in various human tumors compared to benign tissues, highlighting potential as a biomarker for cancer progression and therapeutic target. DNA Ligase I activity alterations are associated with Bloom′s syndrome, a chromosomal breakage disorder, where individuals may exhibit decreased levels of a thermolabile form or a dimeric variant, underscoring DNA Ligase I importance in maintaining genomic stability. anti-DNA Ligase I antibody (F-5) serves as an invaluable tool for researchers investigating DNA repair mechanisms in cancer and genetic disorders.

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DNA Ligase I Antibody (F-5) References:

1. Interaction between PCNA and DNA ligase I is critical for joining of Okazaki fragments and long-patch base-excision repair.  |  Levin, DS., et al. Curr Biol. 10: 919-22. PMID: 10959839
2. DNA ligase I is dephosphorylated during the execution step of etoposide-induced apoptosis.  |  Rossi, R., et al. 2002. Cell Death Differ. 9: 89-90. PMID: 11803377
3. DNA ligase I competes with FEN1 to expand repetitive DNA sequences in vitro.  |  Henricksen, LA., et al. 2002. J Biol Chem. 277: 22361-9. PMID: 11948189
4. DNA ligase I gene expression during differentiation and cell proliferation.  |  Montecucco, A., et al. 1992. Nucleic Acids Res. 20: 6209-14. PMID: 1475182
5. Maternal germline-specific effect of DNA ligase I on CTG/CAG instability.  |  Tomé, S., et al. 2011. Hum Mol Genet. 20: 2131-43. PMID: 21378394
6. Structural alterations of DNA ligase I in Bloom syndrome.  |  Willis, AE., et al. 1987. Proc Natl Acad Sci U S A. 84: 8016-20. PMID: 3479778
7. Specific function of DNA ligase I in simian virus 40 DNA replication by human cell-free extracts is mediated by the amino-terminal non-catalytic domain.  |  Mackenney, VJ., et al. 1997. J Biol Chem. 272: 11550-6. PMID: 9111070
8. An interaction between DNA ligase I and proliferating cell nuclear antigen: implications for Okazaki fragment synthesis and joining.  |  Levin, DS., et al. 1997. Proc Natl Acad Sci U S A. 94: 12863-8. PMID: 9371766
9. DNA ligase I selectively affects DNA synthesis by DNA polymerases delta and epsilon suggesting differential functions in DNA replication and repair.  |  Mossi, R., et al. 1998. J Biol Chem. 273: 14322-30. PMID: 9603940
10. Thermodynamics of human DNA ligase I trimerization and association with DNA polymerase beta.  |  Dimitriadis, EK., et al. 1998. J Biol Chem. 273: 20540-50. PMID: 9685411

The preferred DNA Ligase I antibody is DNA Ligase I Antibody (C-5): sc-271678.