DAZAP1 Antibody (F-5): sc-376208

DAZAP1 Antibody (F-5) is a mouse monoclonal IgG1 antibody that detects DAZAP1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). DAZAP1, or deleted in azoospermia-associated protein 1, is a crucial 407 amino acid RNA-binding protein primarily localized in the nucleus of round spermatids and the cytoplasm of elongated spermatids, playing a vital role in normal spermatogenesis. DAZAP1 contains two RNA recognition motifs, which facilitate interaction with DAZ mRNA, leading to the translation of DAZ proteins essential for germ cell development. Notably, DAZAP1 undergoes phosphorylation, a post-translational modification that causes dissociation from DAZ mRNA, thereby regulating the expression of DAZ proteins and ensuring proper protein translation. This regulatory mechanism is critical, as dysregulation can lead to infertility issues associated with the deletion of DAZ genes in men. Furthermore, DAZAP1 is predominantly expressed in the testis, with lower levels found in the thymus, heart, liver, brain, and pancreas, and exists in two isoforms due to alternative splicing events. Anti-DAZAP1 antibody (F-5) is an invaluable tool for researchers studying spermatogenesis and related disorders, providing insights into the molecular mechanisms underlying male infertility.

The preferred DAZAP1 antibody is DAZAP1 Antibody (D-9): sc-373987.