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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
CYP1A2 Antibody (3B8C1) is a high quality monoclonal CYP1A2 antibody (also designated CYP1A2 antibody) suitable for the detection of the CYP1A2 protein of mouse, rat and human origin. CYP1A2 Antibody (3B8C1) is available as both the non-conjugated anti-CYP1A2 antibody form, as well as multiple conjugated forms of anti-CYP1A2 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. CYP1A2, also called Cytochrome P450 1A2, is a heme-thiolate monooxygenase enzyme involved in the NADPH-dependent electron transport pathway of liver microsomes. A member of the cytochrome P450 family, CYP1A2 oxidizes fatty acids, steroids and xenobiotics. It is also involved in the metabolism of imiprimine, propranol and clozapine. CYP1A2 localizes to the membrane of the endoplasmic reticulum. It is induced by 3-methylcholanthrene, insulin, modafinil and hyperforin and inhibited by many fluoroquinolone antibiotics, caffeine, fluvoxamine and cimetidine. In addition, the involvement of CYP1A2 in the metabolism of estrogen is associated with a reduced risk of breast cancer.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
re: CYP1A2 (3B8C1): sc-53614
- What was the precise antigen used to prepare the MnAb?
What does the MnAb cross-react with?
Posée par: hlll
Thank you for your question. Unfortunately, we do not have any precise information about which antigen was used to develop this monoclonal antibody. It was raised against cytochrome P450 protein of rat origin, but the exact antigen sequence is not known. This antibody is recommended for detection of CYP1A2 of mouse, rat and human origin.
Répondue par: Technical Support
Date de publication: 2019-01-26
I am using CYP1A2 (3B8C1): sc-53614 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?
Posée par: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here:
Rated 3 de
promising IHC stainingI got a promising IHC staining result with DAB in mouse liver.
Date de publication: 2019-11-22
Rated 4 de
Cytoplasmic staining observed in hepatocytesCytoplasmic staining observed in hepatocytes by immunoperoxidase staining of formalin fixed, paraffin embedded (FFPE) human liver tissue. -SCBT QC
Date de publication: 2015-04-27
Rated 4 de
Cytoplasmic localization of the CYP1A2 proteinCytoplasmic localization of the CYP1A2 protein demonstrated in the immunoperoxidase staining of hepatocytes and bile duct cells of formalin fixed, paraffin embedded (FFPE) human liver tissue. -SCBT QC
Date de publication: 2015-03-17
Rated 4 de
Nice band observed via Western Blot usingNice band observed via Western Blot using human liver extract. -SCBT QC
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