Anticorps CYP19 (E-9) recommandé pour la détection de CYP19 d'origine human par WB, IP, IF, IHC(P) et ELISA

Anticorps CYP19 (E-9): sc-374176

Anticorps CYP19 (E-9) est évalué 4.4 de 5 de 5.
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Fiches techniques

    • Anti-L'anticorps CYP19 E-9 est un monoclonal IgG2a (chaîne légère kappa) L'Anticorps CYP19 fourni en 200 µg/ml
    • dirigé contre les acides aminés 204-503 situés au niveau C-terminus de CYP19 d'origine human
    • L'Anticorps CYP19 (E-9) est recommandé pour la détection de CYP19 d'origine human par WB, IP, IF, IHC(P) et ELISA
    • Anti-L'Anticorps CYP19 (E-9) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Voir m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nos recombinants vivement recommandés en alternative des réactifs IgG secondaires anti-souris conventionels.
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de CYP19 (E-9): sc-374176.
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Can CYP19 (E-9): sc-374176 monoclonal antibody be used in IHC with Clarias gariepinus Catfish tissue?

Posée par: Puniragu
Thank you for your question. We do not have any data or product citations using this antibody for detection in any sample of fish origin. If you know the protein sequence of CYP19 in Clarias gariepinus we can assist you by performing sequence analysis against the antigen used to develop this monoclonal antibody. This antibody is raised against amino acids 204-503 of CYP19 of human origin (accession number P11511). Please contact us for further Technical Support.
Répondue par: Technical Support
Date de publication: 2020-04-03

Can CYP19 (E-9): sc-374176 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Posée par: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, CYP19 (E-9): sc-374176 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Répondue par: Technical Support
Date de publication: 2017-02-24
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Rated 5 de 5 de par Worked well on mouse brain lysate Western Blot Was able to detect the correct band on a western blot using mouse brain lysate.
Date de publication: 2018-02-20
Rated 2 de 5 de par unsure about this antibody Honestly, I'm not sure what to think about this antibody. I tried it a number of different tissues (human, cow, and mouse), and am not sure I can detect the correct band. I do, however, get a very bright signal at the wrong molecular weight (~30kDa) that I'm unsure of the identify of.
Date de publication: 2017-12-29
Rated 5 de 5 de par Works very well Works very well in WB, in my cell line. Really happy with it!
Date de publication: 2017-06-05
Rated 5 de 5 de par Works great with porcine samples! I received a sample of this antibody for use in western blotting. It works great with porcine testis samples! It gave one beautiful band of an appropriate molecular weight. It also demonstrated linearity with increasing protein concentrations and the negative control was clear. We are very happy.
Date de publication: 2016-11-30
Rated 5 de 5 de par Great monoclonal antibody Great monoclonal antibody, works perfect for WB with JAR whole cell lysate. -SCBT QC
Date de publication: 2014-05-19
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