CXCR-4 Antibody (12G5): sc-12764

CXCR-4 Antibody (12G5) is a mouse monoclonal IgG2a kappa light chain antibody raised against living CP-MAC-infected SUP-T1 cells, specifically targeting the CXCR-4 protein of human origin, and is validated for applications including immunoprecipitation (IP) and flow cytometry (FCM). CXCR-4, a member of the C-X-C chemokine receptor family, is a G protein-coupled receptor characterized by its seven transmembrane domains and its interaction with stromal cell-derived factor-1 (SDF-1). This receptor plays a pivotal role in cellular signaling and migration, acting as a potent chemoattractant for T cells and monocytes. The CXCR-4/SDF-1 axis is critical for numerous physiological processes, including hematopoiesis, immune response, and organogenesis, as well as pathological conditions like cancer metastasis and immune disorders. CXCR-4 is also the primary co-receptor for T-tropic HIV-1, facilitating viral entry into target cells, while its ligand SDF-1 has been shown to inhibit HIV-1 infection, making this receptor-ligand pair an important focus of antiviral and immunological research. Highly homologous to other IL-8 receptors, CXCR-4 shares approximately 37% sequence identity at the amino acid level, yet exhibits distinct ligand specificity and functional roles. CXCR-4 monoclonal antibody (12G5) is available in non-conjugated form as well as a range of conjugated options, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and Alexa Fluor® conjugates, offering flexibility for diverse research applications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CXCR-4 Antibody (12G5) References:

1. CXCR-4 expression on bone marrow CD34+ cells prior to mobilization can predict mobilization adequacy in patients with hematologic malignancies.  |  Dabusti, M., et al. 2003. J Hematother Stem Cell Res. 12: 425-34. PMID: 12965079
2. Prognostic significance of CXCR-4 expression in oral squamous cell carcinoma.  |  Lee, JI., et al. 2009. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 107: 678-84. PMID: 19272813
3. CXCR-4 and COX-2 expression in basal cell carcinomas and well-differentiated squamous cell carcinomas of the skin; their relationship with tumor invasiveness and histological subtype.  |  Sivrikoz, ON., et al. 2015. Turk Patoloji Derg. 31: 30-5. PMID: 25301050
4. Chemokine CXCR-4 and cyclooxygenase-2 in the pathogenesis of pterygium.  |  Başer, G., et al. 2017. Turk J Med Sci. 47: 1061-1066. PMID: 29152967
5. CXCR-4 expression by circulating endothelial progenitor cells and SDF-1 serum levels are elevated in septic patients.  |  Patry, C., et al. 2018. J Inflamm (Lond). 15: 10. PMID: 29796010
6. Abnormal DNA Methylation Induced by Hyperglycemia Reduces CXCR 4 Gene Expression in CD 34+ Stem Cells.  |  Vigorelli, V., et al. 2019. J Am Heart Assoc. 8: e010012. PMID: 31018749
7. Stem Cell Markers CXCR-4 and CD133 Predict Aggressive Phenotype and Their Double Positivity Indicates Poor Prognosis of Oral Squamous Cell Carcinoma.  |  Caspa Gokulan, R. and Devaraj, H. 2021. Cancers (Basel). 13: PMID: 34885003
8. pH-sensitive nanoliposomes for passive and CXCR-4-mediated marine yessotoxin delivery for cancer therapy.  |  Vieira, AM., et al. 2022. Nanomedicine (Lond). 17: 717-739. PMID: 35481356
9. Molecular effects of genistein, as a potential anticancer agent, on CXCR-4 and VEGF pathway in acute lymphoblastic leukemia.  |  Shahmoradi, M., et al. 2022. Mol Biol Rep. 49: 4161-4170. PMID: 35608747
10. CXCR-4, a chemokine receptor, is overexpressed in and required for proliferation of glioblastoma tumor cells.  |  Sehgal, A., et al. 1998. J Surg Oncol. 69: 99-104. PMID: 9808513

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CXCR-4 Antibody (12G5) has 26 citations in a variety of scientific publications.

Primary antibodies like Anti-CXCR-4 Antibody (12G5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CXCR-4 Antibody (12G5) Specifications

Alternate Product Name: CXCR-4

Product Clone Name: 12G5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: CXCR-4

Epitope Species: human

Product Reactivity/Detection: CXCR-4

Species Reactivity/Detection: human

Additional Mammalian Reactivity: primate

Additional Non-Mammalian Reactivity: N/A

Applications: IP, FCM

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 26 citations in various scientific publications

Available Conjugates/Forms for CXCR-4 Antibody (12G5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, L: Azide-Free, PE: Phycoerythrin

The preferred CXCR-4 antibody is CXCR-4 Antibody (4G10): sc-53534.