Date published: 2025-9-9

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Anticorps CD81 (5A6): sc-23962

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Fiches techniques
  • L'Anticorps CD81 (5A6) est un monoclonal de souris IgG1 κ, cité dans 127 publications, fourni en 200 µg/ml
  • élevé contre des cellules OCI-LY8
  • Anti-L'Anticorps CD81 (5A6) est recommandé pour la détection de CD81 d'origine mouse, rat et human par WB, IP, IF et FCM
  • Anti-L'Anticorps CD81 (5A6) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
  • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
  • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
  • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de CD81 (5A6): sc-23962.
  • m-IgG Fc BP-HRP et 1 BP-HRP">m-IgG1 BP-HRP sont les réactifs de détection secondaire préférés pour CD81 Antibody (5A6) for WB applications. Ces réactifs sont désormais proposés en lots avec CD81 Antibody (5A6)(voir les informations de commande ci-dessous).

ACCÈS RAPIDE AUX LIENS

VOIR ÉGALEMENT...

L'anticorps CD81 (5A6) est un anticorps monoclonal IgG1 kappa à chaîne légère de souris spécifiquement conçu pour cibler la protéine CD81, également connue sous le nom de TAPA-1, un membre éminent de la famille des tétraspanines caractérisé par ses quatre domaines transmembranaires. La protéine CD81 est largement exprimée dans les cellules d'origine hématopoïétique, neuroectodermique et mésenchymateuse, où elle joue un rôle essentiel dans la croissance cellulaire, la transduction des signaux et l'organisation des microdomaines membranaires. Le CD81 subit des modifications post-traductionnelles essentielles, notamment la palmitoylation et la glycosylation, qui sont cruciales pour sa localisation, sa stabilité et ses interactions avec d'autres protéines membranaires telles que le CD37, le CD53, le CD19, le CD21 et les antigènes du CMH de classe II. L'anticorps anti-CD81 (5A6) a été développé à partir de la lignée cellulaire de lymphome B OCI-LY8, assurant une détection efficace de CD81 chez la souris, le rat et l'homme par des applications telles que le western blotting (WB), l'immunoprécipitation (IP), l'immunofluorescence (IF) et la cytométrie de flux (FCM). Disponible sous des formes conjuguées, y compris la peroxydase de raifort (HRP), la phycoérythrine (PE), l'isothiocyanate de fluorescéine (FITC) et divers conjugués Alexa Fluor®, et sous des formes non conjuguées, l'anticorps monoclonal CD81 (5A6) offre des options polyvalentes pour répondre à divers besoins expérimentaux. En ciblant CD81, les chercheurs peuvent étudier des processus critiques tels que l'activation des cellules immunitaires, les mécanismes d'entrée des virus et les voies de signalisation complexes qui régissent les fonctions cellulaires. Les modifications post-traductionnelles de CD81 sont particulièrement importantes pour comprendre le rôle de CD81 dans des conditions pathologiques telles que les métastases cancéreuses et les troubles immunitaires, ce qui fait de l'anticorps anti-CD81 (5A6) un outil essentiel pour la recherche fondamentale et appliquée.

Pour la Recherche Uniquement. Non destiné à un usage diagnostique ou thérapeutique.

Alexa Fluor® est une marque déposée de Molecular Probes Inc., OR., USA

LI-COR® et Odyssey® sont marques déposées de LI-COR Biosciences

Informations pour la commande

Nom du produitRef. CatalogueCOND.Prix HTQTÉFavoris

Anticorps CD81 (5A6)

sc-23962
200 µg/ml
$316.00

CD81 (5A6): m-IgG Fc BP-HRP Kit

sc-526643
200 µg Ab; 10 µg BP
$354.00

CD81 (5A6): m-IgG1 BP-HRP Kit

sc-532016
200 µg Ab; 20 µg BP
$354.00

Anticorps CD81 (5A6) AC

sc-23962 AC
500 µg/ml, 25% agarose
$416.00

Anticorps CD81 (5A6) HRP

sc-23962 HRP
200 µg/ml
$316.00

Anticorps CD81 (5A6) FITC

sc-23962 FITC
200 µg/ml
$330.00

Anticorps CD81 (5A6) PE

sc-23962 PE
200 µg/ml
$343.00

Anticorps CD81 (5A6) Alexa Fluor® 488

sc-23962 AF488
200 µg/ml
$357.00

Anticorps CD81 (5A6) Alexa Fluor® 546

sc-23962 AF546
200 µg/ml
$357.00

Anticorps CD81 (5A6) Alexa Fluor® 594

sc-23962 AF594
200 µg/ml
$357.00

Anticorps CD81 (5A6) Alexa Fluor® 647

sc-23962 AF647
200 µg/ml
$357.00

Anticorps CD81 (5A6) Alexa Fluor® 680

sc-23962 AF680
200 µg/ml
$357.00

Anticorps CD81 (5A6) Alexa Fluor® 790

sc-23962 AF790
200 µg/ml
$357.00

What is the isoelectric point for CD-81 antibody?

Posée par: Sumita
Thank you for your question. We do not characterize the isoelectric point of our antibodies.
Répondue par: BlakeJ
Date de publication: 2025-07-23

Hello, I have used the CD81 antibody for western blot at 1:1500 in 5% MILK on exosomes from human plasma. Do you know why I get two bands, one at 23 kDa and another one at 48 kDa? Many thanks Juliana

Posée par: Juliana Redondo
Thank you for your question. The expected molecular weight for CD81 is ~ 22-26 kDa. We have not tested this antibody specifically in human plasma, and have not observed a higher molecular weight signal at 48 kDa in the cell lines tested. CD81 has been reported to dimerize (PMID: 20375010) and that may be a consideration. We would also recommend including a secondary antibody control, where you omit the primary antibody from the incubation, to confirm if the higher molecular weight band might be due to non-specific binding of the secondary antibody alone.
Répondue par: Technical Support
Date de publication: 2017-10-16

Hi, I have used this antibody at 1:100 concentration on a western blot (10% SDS-PAGE gel) loading some exosomal protein. Could you explain please why I get not only the 26 kDa band, but also a band at about 50 kDa? Thank you.

Posée par: VivSal
Thank you for your question. We have not tested this antibody on exosomal protein, or observed a 50 kDa band in Western blot. We would recommend including a secondary antibody control (omit primary antibody) to identify if the 50 kDa band may be a result of non-specific binding of the secondary antibody alone. It could also be your secondary antibody binding to heavy chain IgG in your samples. Below is a link to our recommended protocol for Western blotting. Please contact our Technical Service Department if for additional support with troubleshooting the antibody. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Répondue par: Technical Support
Date de publication: 2017-10-16

I have tried using this antibody on a western blot (after running an SDS-PAGE gel) with a homemade K562 lysate as control and did not get a signal at 1:100 dilution. I tried it 3 times previously on other cell lines at 1:200 w/no success. Any suggestions?

Posée par: Tali
Thank you for your question. If you have no signal, we would recommend verifying that your anti-mouse secondary is working well. We would also recommend including a loading control antibody (e.g., Actin or GAPDH) to ensure that your transfer conditions are good, as well as your signal detection conditions (e.g., chemiluminescence or other). We have successfully used this antibody for WB at a dilution of 1:200, loading 50 µg of K-562 whole cell lysate. A link to our complete recommended protocol is provided below. If you continue to have negative results, please contact our Technical Service Department so we can provide further troubleshooting support. Santa Cruz Biotechnology does warranty our antibodies for one year from the date of purchase. If we are unable to successfully troubleshoot your application we will send you a replacement antibody free of charge, or issue a credit or refund if that is not possible https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting .
Répondue par: Technical Support
Date de publication: 2017-04-26

What concentration would you recommend using SC-20048 at, to detect a protein in paraffin-embedded human tissue? We have used 1:1000 successfully for western blots. Please can you reply at your earliest convenience.

Posée par: Deborah Goberdhan
Thank you for your question! We recommend a starting dilution of 1:50 for IHC on paraffin embedded tissue sections. This is a starting dilution, so it is possible that you can use it much more diluted! We also recommend performing an antigen retrieval step, ideally sodium citrate with heat. You can find our recommended protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Répondue par: Tech Service
Date de publication: 2017-02-22

1, What concentration do you recommend using SC-23962 on paraffin embedded human tissue sections? We have this antibody, so would like to try. ; 2. Do you think that SC-23962 FITC would work better and at what conc. for same application, ie IHC(P)?

Posée par: Deborah Goberdhan
Thank you for your question! We recommend a starting dilution of 1:50 for IHC on paraffin embedded tissue sections. We do recommend performing an antigen retrieval step. You can find our recommended protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining You can use the FITC conjugated form, although we do not generally use FITC conjugated antibodies for IHC in house, so I do not have test results for this. If you wish to try it, I would stick with the 1:50 starting dilution as well. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Répondue par: Tech Service
Date de publication: 2017-02-22

Can I use this antibody for staining of mouse tissue, since it is a mouse antibody?

Posée par: Anna99
Yes. CD81 Antibody (5A6): sc-23962 is available conjugated to either phycoerythrin (sc-23962 PE) or fluoroscein (sc-23962 FITC), 200 µg/ml, for IF, IHC(P) and FCM.
Répondue par: Tech Service 5
Date de publication: 2017-01-05
  • y_2025, m_8, d_28, h_5CST
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Rated 4 de 5 de par Great antibodyI request a sample of the antibody and it worked well!
Date de publication: 2023-07-31
Rated 4 de 5 de par buon funzionamentoHo effettuato un solo esperimento per verificare la reattività nel cane e ha funzionato
Date de publication: 2023-07-14
Rated 1 de 5 de par WBでバンドが検出できませんでした。エクソソームサンプルでCD81のバンドが全く検出できませんでした。同じサンプルで他のCD81抗体を使うとCD81を検出できたのでサンプルは問題ないと考えています。
Date de publication: 2021-09-09
Rated 2 de 5 de par No band in Western blotI cannot see any band in Western blot after a nice transfer (all MW completely transfered; blotting using 1:200 of this antibody and 2nd antibody sc-516102 at 1:1000) of exosomes isolated by ultracentrifugation and further separated by density centrifugation. Perhaps I have low concentration of exosomes, but I expected to get at least a faint band.
Date de publication: 2021-05-25
Rated 5 de 5 de par Works well in western blotThe antibody detected CD81 in Mesenchymal stem cells at the expected molecular size.
Date de publication: 2020-09-18
Rated 5 de 5 de par Tested on HFFF by FACSThis antibody was tested on HFFF cells by FACS and shown a very strong signal, even at dilution 1:100
Date de publication: 2018-11-19
Rated 5 de 5 de par Excellent antibody for ICC stainingThis antibody is gorgeous for ICC staining, please see the attached confocal image. Highly recommended. I tried 1:50 dilution for the first time, and you may further dilute it to 1:100 or even 1:200 to decrease the background a little bit, and it should work even better. I feel the overall quality of SCBT antibodies is getting much better,
Date de publication: 2018-11-08
Rated 5 de 5 de par likeMembrane localization observed by Immunofluorescence staining of methanol-fixed embedded HeLa cells, also good for staining.
Date de publication: 2017-05-07
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Anticorps CD81 (5A6) est évalué 4.1 de 5 de 14.
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