Anticorps CD73 (IE9) recommandé pour la détection de CD73 d'origine human par WB, IP, IF et IHC(P)

Anticorps CD73 (IE9): sc-32299

Anticorps CD73 (IE9) est évalué 4.9 de 5 de 8.
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Fiches techniques

    • L'Anticorps CD73 (IE9) est un monoclonal de souris IgG3 κ, cité dans 29 publications, fourni en 200 µg/ml
    • raised against human CD73 purified from placenta
    • recommandé pour la détection de CD73 d'origine human par WB, IP, IF et IHC(P); non cross-reactif avec mouse CD73
    • Voir CD73 (D-12): sc-398260 pour d'autres anticorps conjugués CD73, dont AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 et 790.
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de CD73 (IE9): sc-32299.

Hi, I would like to know if it is possible to have the product sc-32299 in a carrier-free version (without gelatin)? Thank you

Posée par: Anonyme
Thank you for your question. Yes, this can be provided carrier-free (without gelatin) as a special order. Please contact our Special Orders Department to inquire about availability and to receive a quote. Please call our Technical Service Department, or email <[email protected]>.
Répondue par: Technical Support
Date de publication: 2019-12-07

Hello: Was CD73 Antibody (IE9): sc-32299 tested on rat tissues? It was found to inhibit the catalytic activity of CD73 (in http://www.jimmunol.org/content/191/8/4165.long). This would be great if I could use it in rat tissue homogenates? thanks, Fran.

Posée par: FranVdB
Thank you for your question. We do not have any data indicating that this mouse monoclonal antibody, CD73 (IE9): sc-32299, is reactive with rat CD73. We recommend the antibody for detection of CD73 of human origin only. In the publication you have cited, the authors specifically mention that IE9 clone "failed to detect the murine CD73 variant ".
Répondue par: Technical Support
Date de publication: 2017-06-28

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Posée par: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Répondue par: Tech Service 3
Date de publication: 2016-12-23
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Rated 5 de 5 de par Works well for human mesenchymal Stem cells Received sample for this 2 months ago. Worked pretty well with human BMMSCs at 1:50. Definitely going to purchase
Date de publication: 2020-03-10
Rated 4 de 5 de par good used dilution 1: 150 overnight for immunofluorescence
Date de publication: 2018-04-10
Rated 5 de 5 de par Works well in Western This is an excellent antibody for use in Western Blot - good band strength and clarity with little to no background.
Date de publication: 2016-12-27
Rated 5 de 5 de par Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the CD73 protein via Western Blot analysis. -SCBT QC
Date de publication: 2015-05-16
Rated 5 de 5 de par Tested for western blotting with MB Tested for western blotting with MB-MDA-231, MB-MDA-453, MB-MDA-468, T47D, and MCF-7 cells, and breast cancer tissue extracts. -SCBT Publication Review
Date de publication: 2015-04-09
Rated 5 de 5 de par IHC: acetone fixed ovarian cancer cryosections IHC: acetone fixed ovarian cancer cryosections. -SCBT Publication Review
Date de publication: 2015-01-24
Rated 5 de 5 de par Clean band in human recombinant CD73 via Clean band in human recombinant CD73 via western blotting. -SCBT QC
Date de publication: 2015-01-23
Rated 5 de 5 de par Published IHC data with human trabecular Published IHC data with human trabecular bone sections. -SCBT Publication Review
Date de publication: 2014-09-25
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