CD73 Antibody (2B6): sc-130006

CD73 Antibody (2B6) is a mouse monoclonal IgG2b antibody that detects CD73 of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-CD73 antibody (2B6) is available as the non-conjugated form. CD73, also known as 5′-nucleotidase, E5NT, NT, NT5, NTE, eN, and eNT, is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that plays a crucial role in the regulation of extracellular nucleotide metabolism by catalyzing the dephosphorylation of purine and pyrimidine nucleotides into their corresponding bioactive nucleosides. This enzymatic activity is vital for maintaining immune homeostasis and modulating inflammatory responses, as CD73 influences the availability of nucleosides that can act on various receptors, thereby affecting cell signaling pathways. CD73 is structurally characterized as a dimer of two identical subunits, which relies on its GPI anchor for attachment to the external face of the plasma membrane. CD73′s location and anchoring mechanism are significant because these features facilitate interaction with other cell surface proteins and enable mediation of co-stimulatory signals during T cell activation. CD73 exhibits diverse biological functions through differential regulation in various cell types, including endothelial cells, subpopulations of B and T cells, germinal center follicular dendritic cells, and thymic medullary reticular fibroblasts. Notably, CD73 neutralization by IgG can disrupt lymphocyte adhesion to endothelial cells and inhibit the aggregation of germinal center B cells and follicular dendritic cells, highlighting CD73′s importance in immune cell interactions. Furthermore, targeting lymphocyte CD73 can lead to shedding and subsequent tyrosine phosphorylation of associated proteins, underscoring CD73′s multifaceted role in immune regulation.

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CD73 Antibody (2B6) References:

1. The roles of CD73 in cancer.  |  Gao, ZW., et al. 2014. Biomed Res Int. 2014: 460654. PMID: 25126561
2. Immune profiling of human tumors identifies CD73 as a combinatorial target in glioblastoma.  |  Goswami, S., et al. 2020. Nat Med. 26: 39-46. PMID: 31873309
3. CD4+FoxP3+CD73+ regulatory T cell promotes cardiac healing post-myocardial infarction.  |  Zhuang, R., et al. 2022. Theranostics. 12: 2707-2721. PMID: 35401839
4. MET-Induced CD73 Restrains STING-Mediated Immunogenicity of EGFR-Mutant Lung Cancer.  |  Yoshida, R., et al. 2022. Cancer Res. 82: 4079-4092. PMID: 36066413
5. CD73-Dependent Adenosine Signaling through Adora2b Drives Immunosuppression in Ductal Pancreatic Cancer.  |  Faraoni, EY., et al. 2023. Cancer Res. 83: 1111-1127. PMID: 36720042
6. CD73 mitigates hepatic damage in alcoholic steatohepatitis by regulating PI3K/AKT-mediated hepatocyte pyroptosis.  |  Zhu, H., et al. 2023. Biochem Pharmacol. 215: 115753. PMID: 37611643
7. Impact of CRISPR/Cas9-Mediated CD73 Knockout in Pancreatic Cancer.  |  Zhang, J., et al. 2023. Cancers (Basel). 15: PMID: 37835536
8. CD73 controls Myosin II-driven invasion, metastasis, and immunosuppression in amoeboid pancreatic cancer cells.  |  Samain, R., et al. 2023. Sci Adv. 9: eadi0244. PMID: 37851808
9. The hypoxia-regulated ectonucleotidase CD73 is a host determinant of HIV latency.  |  Sperber, HS., et al. 2023. Cell Rep. 42: 113285. PMID: 37910505
10. Novel CD73 Inhibitors for Treating Cancer.  |  Sabnis, RW. 2024. ACS Med Chem Lett. 15: 571-572. PMID: 38746902

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD73 Antibody (2B6) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-CD73 Antibody (2B6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD73 Antibody (2B6) Specifications

Alternate Product Name: 5'-nucleotidase

Product Clone Name: 2B6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids: 27-252

Epitope Target: CD73

Epitope Species: human

Product Reactivity/Detection: CD73

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for CD73 Antibody (2B6): N/A

The preferred CD73 antibody is CD73 Antibody (IE9): sc-32299.