Les Produits Chimiques ChemCruz™
CD71 Inhibiteurs pour l'analyse fonctionnelle des réponses cellulaires de CD71
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
ACCÈS RAPIDE AUX LIENS
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
CD71 Antibody (3B8 2A1) is a high quality monoclonal CD71 antibody (also designated p90 antibody, TFRC antibody, TfR1 antibody or Transferrin antibody) suitable for the detection of the CD71 protein of human origin. CD71 Antibody (3B8 2A1) is available as both the non-conjugated anti-CD71 antibody form, as well as multiple conjugated forms of anti-CD71 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. CD71, also known as the transferrin receptor (TFR), is a type II membrane glycoprotein that exists as a disulfide-linked homodimer of two identical subunits. CD71 binds to two molecules of transferrin and a serum iron-transport protein, and directs the cellular uptake of iron via receptor-mediated endocytosis. CD71 is expressed, typically at high levels, on all proliferating cells, reticulocytes and erythroid precursors. It is not expressed on resting leukocytes, but is upregulated upon activation of lymphocytes, monocytes and macrophages. CD71 is also found on most dividing cells and on brain endothelium. A second transferrin receptor, TFR2, also mediates the uptake of transferrin-bound iron. TFR2 is a two-subunit homodimer and is highly expressed in liver as well as in hepatocytes and erythroid precursors. Mutations in the TFR2 gene result in hereditary hemochromatosis type III (HFE3), an iron overloading disorder predominant in Caucasians.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Does this antibody [(3B8 2A1), sc-32272] block binding of Transferrin to CD71?
Posée par: PRCH
Thank you for your question. This antibody has not been tested for functional or blocking activity.
Répondue par: Tech Service
Date de publication: 2019-11-04
What is the recommended fixation method for IF with CD71 Antibody (3B8 2A1): sc-32272?
Posée par: jenni
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Rated 2 de
It does not work on mouse cells.It does not work on mouse cells (A20), but it is fair, as Santa Cruz recommended this antibody for detection of CD71 of human origin. I just gave it a try anyway.
Date de publication: 2018-03-08
Rated 3 de
Good Flow, Poor IFAntibody worked well for FC at 1ug/1 million cells. Seemed to show some surface staining of T cells via IF at a 1:100 dilution.
Date de publication: 2017-11-20
Rated 5 de
Good FeatureUsed for IF on human tissue, clean signal, good antibody.
Date de publication: 2017-10-05
Rated 5 de
WB for TFRCVery clear western blot data; strong signal with low background；test at 1:300
Date de publication: 2017-09-14
Rated 5 de
Yubin Feng par
good featuresuse in NB4 cells ,the trips is very abvious,thank you for your free sample
Date de publication: 2017-09-06
Rated 4 de
Date de publication: 2017-08-01
Rated 5 de
TFRCThe TFRC Antibody works very well for Western blot applications, showing strong and clear banding with minimal background noise present
Date de publication: 2017-07-31
Rated 5 de
suitable for FCMstrong signals when 1:50 dilution for FCM in Jurkat and PC-3 cells.
Votre abonnement par e-mail à Nouvelles et Annonces a été reçu. En vous inscrivant, vous indiquez que vous avez lu et accepté les termes de notre politique de confidentialité . Vous avez le droit de vous désabonner à tout moment en cliquant sur le lien Se désinscrire dans les e-mails que vous recevez.
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn