Anticorps CD47 (B6H12) recommandé pour la détection de the immunoglobulin domain of CD47 d'origine et human par WB, IP, IF, IHC(P) et FCM

Anticorps CD47 (B6H12): sc-12730

Anticorps CD47 (B6H12) est évalué 4.1 de 5 de 14.
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Fiches techniques

    • Anti-L'anticorps CD47 B6H12 est un monoclonal IgG1 κ L'Anticorps CD47, cité dans 34 publications, fourni en 200 µg/ml
    • raised against intact CD47 purified from placenta
    • Anti-L'Anticorps CD47 (B6H12) est recommandé pour la détection de the immunoglobulin domain of CD47 d'origine et human par WB, IP, IF, IHC(P) et FCM
    • L'Anticorps CD47 (B6H12) est disponible conjugué à l'agarose pour IP; et soit à la phycoerythrin ou FITC our IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de CD47 (B6H12): sc-12730.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for CD47 Antibody (B6H12) for WB and IHC(P) applications. This reagent is now offered in a bundle with CD47 Antibody (B6H12) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Hi, the concentration of the mAb, catalogue no. sc-12730 is stated as 100 microgram/mL. Can custom amount be ordered at higher concentration, what is the maximum amount and concentration?

Posée par: Anonyme
Thank you for your question. Please contact our Special Orders Department to inquire about availability and to receive a quote for a custom concentration of sc-12730. Please call our Technical Service Department, or email <[email protected]>.
Répondue par: Tech Service 1
Date de publication: 2020-11-12

Is there an SDS for this product?

Posée par: BonnieK
Thank you for your question. Our antibody products are classified as non-hazardous items, so there is no SDS documentation for them.
Répondue par: Tech Support Europe
Date de publication: 2020-08-19

A desperate client of mine with a dog with an intestinal tumor, cited a Standford U research that used CD47 for shrinking tumors. Is it available for sale? Dose? Cost?

Posée par: Dr B
Thank you for your question. Our antibodies are for research use only. They are not suitable for diagnostic or therapeutic use. Best of luck with your research.
Répondue par: Technical Support
Date de publication: 2020-05-18

Bonjour , Serait-il possible de tester un échantillon de cet anticorps avant de le commander ? Merci

Posée par: Cath54
Thank you for your question. Please contact Technical Service or your local distributor for information on how to receive a free 10 ug sample of this monoclonal antibody.
Répondue par: Tech Service
Date de publication: 2019-12-04

Does this antibody detect mouse CD47 in western blot? I thought it only detects human CD47 previously. You had MIAP301 for mouse. What have you changed in B6H12 antibody that it's able to detect mouse CD47?

Posée par: Kedar Ghimire
Thank you for your question. No, this antibody is specific for human CD47. We added mouse to the species reactivity based on a product citation using this antibody. However, we are going to remove mouse and only recommend this for human, unless we are able to get data in a mouse sample with this clone.
Répondue par: Technical Support
Date de publication: 2019-04-13

Hi Can i use this antibody for flow Cytometric and western blot assay for the detection of CD47 on erythrocytes of Rhesus and Cynomolgus macaques?

Posée par: ashi_10123
Thank you for your question. This CD47 monoclonal antibody has been tested and published for Western blot and flow cytometry in human samples. We are not aware of any data or product citations in Rhesus and Cynomolgus macaques. Although, this CD47 clone B6H12 is recommended for detection of CD47 in primates, so it may also be suitable for these species.
Répondue par: Technical Support
Date de publication: 2019-03-16

Is it know which epitope this antibody recognizes? Was it raised against a specific peptide (which one?) or fulll length protein (recombinant or purified?)? Thank you!

Posée par: babs440
Thank you for your question. This mouse monoclonal antibody was raised against intact CD47 purified from placenta. The exact epitope recognized by the monoclonal antibody has not been determined.
Répondue par: Technical Support
Date de publication: 2019-01-17

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Posée par: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody is currently available conjugated to phycoerythrin (sc-12730 PE) or fluoroscein (sc-12730 FITC), Alexa Fluor® 488 (sc-12730 AF488) or Alexa Fluor® 647 (sc-12730 AF647). Please contact Technical Service if you have further questions concerning this product.
Répondue par: Tech Service 9
Date de publication: 2016-12-30
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Rated 4 de 5 de par Works for FCM The CD47 antibody works for flow cytometry on HeLa cells.
Date de publication: 2019-05-16
Rated 3 de 5 de par Western blot This antibody didn't had good performance in western blot (applied in gastric cancer cell line: AGS, MKN45), sometimes weak band and high background.
Date de publication: 2017-11-17
Rated 1 de 5 de par it doesn't work in WB I have tested this antibody for WB (1:200) on whole cell lysates from HEK293, Jurkat and U251. Despite the datasheet shows a clear band around 49kDa, I could not detect any band in any of these cell lines. We tested several conditions and we can confirm the antibody doesn't work.
Date de publication: 2017-10-19
Rated 4 de 5 de par Good antibody, strong signal Got this antibody as a trial antibody. Worked well on human airway epithelial cells (CFBE cells)
Date de publication: 2017-10-09
Rated 5 de 5 de par Good for western blot in human cell lines I used this antibody before 3 days ago.The product shows good quality. The samples are protein of U937 cells treated with PMA and MDA-MB-231. Bands are clear in low background.
Date de publication: 2017-08-28
Rated 5 de 5 de par good IHC staining Good cytoplasmic staining with paraffin-embedded human adrenal gland tissue.
Date de publication: 2017-05-28
Rated 5 de 5 de par perfect! The product shows good quality for western blot with human platelet extract
Date de publication: 2017-05-23
Rated 5 de 5 de par Conjugates great for IF! Dual staining in IF is beautiful when using the directly conjugated form of this antibody.
Date de publication: 2017-01-11
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