Anticorps CD3 (5B2) recommandé pour la détection de CD3 d'origine mouse, rat et human par IP, IF et FCM

Anticorps CD3 (5B2): sc-70626

Anticorps CD3 (5B2) est évalué 5.0 de 5 de 2.
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Fiches techniques

    • L'anticorps CD3 5B2 est un monoclonal IgG3 de souris fourni en 100 µg/ml
    • raised against human peripheral lymphocytes
    • recommandé pour la détection de CD3 d'origine mouse, rat et human par IP, IF et FCM
    • disponible conjugué à la FITC pour IF, IHC(P) et FCM
    • Voir CD3 (PC3/188A): sc-20047 pour d'autres anticorps conjugués CD3, dont AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 et 790.

Should I permeabilize the cells if I want use the 5B2 for FACS analysis?

Posée par: Elenarb000
Thank you for your question. Yes, we recommend fixing and permeabilizing when using this mouse monoclonal antibody, CD3 (5B2): sc-70626, for flow cytometry (FCM) for FACS applications. A link to our recommended protocol for FCM is provided below. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Répondue par: Technical Support
Date de publication: 2017-09-20

I am using CD3 (5B2): sc-70626 monoclonal antibody for immunoprecipitation, how much antibody should I use?

Posée par: DefinitelyNotMatt
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Répondue par: Technical Support
Date de publication: 2017-04-05
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Rated 5 de 5 de par IF publication with rat C6 cells IF publication with rat C6 cells. -SCBT Publication Review
Date de publication: 2015-05-01
Rated 5 de 5 de par CD3 (5B2) was used successfully in mouse D1 and D1 CD3 (5B2) was used successfully in mouse D1 and D1-CD4 T cells for immunoprecipitation. -SCBT Publication Review
Date de publication: 2013-04-25
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