caspase-3 p17 Antibody (B-4): sc-271028

caspase-3 p17 Antibody (B-4) is a mouse monoclonal IgG2a kappa light chain antibody engineered to detect the p17 subunit of caspase-3, also known as apopain, SCA-1, Yama, and CPP32. Anti-caspase-3 p17 antibody (B-4) targets amino acids 56-104 near the N-terminus of human caspase-3 p17, ensuring precise recognition of both the p17 subunit and the full-length precursor of caspase-3 in human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Caspase-3 is a vital aspartate-specific cysteine protease within the ICE subfamily of caspases, playing a crucial role in the execution phase of apoptosis, the programmed cell death process essential for development, tissue homeostasis, and the removal of damaged or unwanted cells. Structurally, caspase-3 is synthesized as an inactive proenzyme, procaspase-3, which undergoes proteolytic cleavage to form the active enzyme composed of two p17 and two p11 subunits, creating a heterotetramer essential for enzymatic activity. This structural configuration allows caspase-3 to effectively cleave key substrates such as PARP, facilitating the orderly disassembly of cellular components during apoptosis. Active caspase-3 can activate downstream caspases like caspase-6, -7, and -9, thereby amplifying the apoptotic signal and ensuring completion of the cell death process. The precise structural organization of caspase-3 is critical for function, enabling rapid response to apoptotic signals within the cytoplasm, where caspase-3 is highly expressed in vital organs including lung, spleen, heart, liver, kidney, and immune cells. caspase-3 p17 monoclonal antibody (B-4) serves as an indispensable tool for researchers investigating molecular mechanisms of apoptosis, cancer progression, neurodegenerative diseases, and other conditions where programmed cell death is key. Anti-caspase-3 p17 antibody (B-4) demonstrates high specificity and versatility across multiple applications, making this reagent ideal for advancing studies in cellular biology and related fields.

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caspase-3 p17 Antibody (B-4) References:

1. Apoptosis-induced release of mature sterol regulatory element-binding proteins activates sterol-responsive genes.  |  Higgins, ME. and Ioannou, YA. 2001. J Lipid Res. 42: 1939-46. PMID: 11734566
2. Apoptosis and proliferation markers in diffusely infiltrating astrocytomas: profiling of 17 molecules.  |  Liu, X., et al. 2006. J Neuropathol Exp Neurol. 65: 905-13. PMID: 16957584
3. A primate-specific acceleration in the evolution of the caspase-dependent apoptosis pathway.  |  Vallender, EJ. and Lahn, BT. 2006. Hum Mol Genet. 15: 3034-40. PMID: 16980329
4. 3-(3,4,5-Trimethoxyphenyl)-1-oxo-2-propene: a novel pharmacophore displaying potent multidrug resistance reversal and selective cytotoxicity.  |  Das, U., et al. 2007. Bioorg Med Chem. 15: 3373-80. PMID: 17383883
5. Alterations in mRNA expression of apoptosis-related genes BCL2, BAX, FAS, caspase-3, and the novel member BCL2L12 after treatment of human leukemic cell line HL60 with the antineoplastic agent etoposide.  |  Floros, KV., et al. 2006. Ann N Y Acad Sci. 1090: 89-97. PMID: 17384250
6. Caspase-3 activation, Bcl-2 contents, and soluble FAS-ligand are not related to the inflammatory marker profile in patients with sepsis and septic shock.  |  Kriebel, F., et al. 2006. Ann N Y Acad Sci. 1090: 168-76. PMID: 17384259
7. Role of ATP in trauma-associated cytokine release and apoptosis by P2X7 ion channel stimulation.  |  Schneider, EM., et al. 2006. Ann N Y Acad Sci. 1090: 245-52. PMID: 17384268
8. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis.  |  Nicholson, DW., et al. 1995. Nature. 376: 37-43. PMID: 7596430
9. Caspases: the executioners of apoptosis.  |  Cohen, GM. 1997. Biochem J. 326 (Pt 1): 1-16. PMID: 9337844

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-caspase-3 p17 Antibody (B-4) has 72 citations in a variety of scientific publications.

Primary antibodies like Anti-caspase-3 p17 Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

caspase-3 p17 Antibody (B-4) Specifications

Alternate Product Name: caspase-3 p17

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 56-104

Epitope Target: caspase-3 p17

Epitope Species: human

Product Reactivity/Detection: p17 subunit and full length precursor of caspase-3

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 72 citations in various scientific publications

Available Conjugates/Forms for caspase-3 p17 Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred caspase-3 antibody is caspase-3 Antibody (E-8): sc-7272.