Calregulin Antibody (H-10): sc-166839
- Calregulin Antibody (H-10) is a mouse monoclonal IgG2a κ, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 248-417 of Calregulin of human origin
- recommended for detection of Calregulin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- See Calregulin (F-4): sc-373863 for Calregulin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Calregulin Antibody (H-10) for WB and IHC(P) applications. These reagents are now offered in bundles with Calregulin Antibody (H-10) (see ordering information below).
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Calregulin Antibody (H-10) is a mouse monoclonal IgG2a antibody that detects calregulin in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Calregulin, also known as calreticulin, is a multifunctional calcium-binding protein predominantly located in the lumen of the endoplasmic reticulum, where calregulin plays a crucial role in the proper folding and assembly of glycoproteins. By binding to newly synthesized glycoproteins, calregulin assists in their maturation and helps retain misfolded proteins, thereby preventing their export from the endoplasmic reticulum. This function is vital for maintaining cellular homeostasis and ensuring that only properly folded proteins are transported to their final destinations, which is essential for normal cellular function and response to stress. Calregulin′s ability to bind calcium through low- and high-affinity sites further underscores calregulin′s importance in cellular signaling and homeostasis. With broad species reactivity and versatility in various applications, anti-calregulin antibody (H-10) is an invaluable tool for researchers studying protein folding, endoplasmic reticulum stress, and related cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Calregulin Antibody (H-10) References:
- Calreticulin in cardiac development and pathology. | Michalak, M., et al. 2002. Biochim Biophys Acta. 1600: 32-7. PMID: 12445456
- Calreticulin is an upstream regulator of calcineurin. | Lynch, J. and Michalak, M. 2003. Biochem Biophys Res Commun. 311: 1173-9. PMID: 14623303
- Transcriptional control of the calreticulin gene in health and disease. | Qiu, Y. and Michalak, M. 2009. Int J Biochem Cell Biol. 41: 531-8. PMID: 18765291
- Somatic mutations of calreticulin in myeloproliferative neoplasms and myelodysplastic/myeloproliferative neoplasms. | Malcovati, L., et al. 2014. Haematologica. 99: 1650-2. PMID: 25420280
- The ever-expanding immunomodulatory role of calreticulin in cancer immunity. | de Bruyn, M., et al. 2015. Front Oncol. 5: 35. PMID: 25750898
- Calreticulin in the Heart: From Embryological Development to Cardiac Pathology. | Martinho-Dias, D., et al. 2016. Curr Mol Med. 16: 12-22. PMID: 26695698
- Mutant calreticulin in myeloproliferative neoplasms. | How, J., et al. 2019. Blood. 134: 2242-2248. PMID: 31562135
- The role of calreticulin mutations in myeloproliferative neoplasms. | Araki, M. and Komatsu, N. 2020. Int J Hematol. 111: 200-205. PMID: 31848992
- Calreticulin mutations affect its chaperone function and perturb the glycoproteome. | Schürch, PM., et al. 2022. Cell Rep. 41: 111689. PMID: 36417879
- Development of a radioimmunoassay for quantitation of calregulin in bovine tissues. | Khanna, NC. and Waisman, DM. 1986. Biochemistry. 25: 1078-82. PMID: 3964661
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Calregulin Antibody (H-10) | sc-166839 | 200 µg/ml | $322.00 | |||
Calregulin Antibody (H-10): m-IgG Fc BP-HRP Bundle | sc-537535 | 200 µg Ab; 10 µg BP | $361.00 | |||
Calregulin Antibody (H-10): m-IgGκ BP-HRP Bundle | sc-534831 | 200 µg Ab; 40 µg BP | $361.00 |