Anticorps Calpain 2 (E-10) recommandé pour la détection de Calpain 2 precursor d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA

Anticorps Calpain 2 (E-10): sc-373966

Anticorps Calpain 2 (E-10) est évalué 4.8 de 5 de 10.
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Fiches techniques

    • L'Anticorps Calpain 2 (E-10) est un monoclonal de souris IgG1 κ, cité dans 10 publications, fourni en 200 µg/ml
    • spécifique d'un épitope situé entre les acides aminés 2-27 au niveau N-terminus de Calpain 2 d'origine human
    • Anti-L'Anticorps Calpain 2 (E-10) est recommandé pour la détection de Calpain 2 precursor d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA
    • Anti-L'Anticorps Calpain 2 (E-10) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de Calpain 2 (E-10): sc-373966.

Which tissue or cell can be a positive control for calpain2 IHC ?

Posée par: hexl7
Thank you for your question. Bronchus tissue, fallopian tube, gall bladder or rectum tissues are all good positive controls for this protein for IHC.
Répondue par: Tech Service
Date de publication: 2019-04-22

Was the antibody immunized against isoform 1 or 2 of calpain-2? Isoform 2 lacks the first 78 amino acids of isoform 1.

Posée par: Claudia_NeuroGU
Thank you for your question. Calpain 2 Antibody (E-10): sc-373966 is specific for an epitope mapping between amino acids 2-27 at the N-terminus of Calpain 2 of human origin. The immunogenic sequence is AGIAAKLAK DREAAEGLGS HDRAIKY. Since amino acids 1-78 are missing in isoform P17655-2, this isoform is not recognized by Calpain 2 Antibody (E-10): sc-373966 or Calpain 2 Antibody (F-11): sc-373967, which has been raised against the same range of amino acids.
Répondue par: Technical Support Europe
Date de publication: 2019-01-14

What application is the blocking peptide sc-373966 P appropriate for?

Posée par: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Répondue par: Technical Support
Date de publication: 2017-02-24
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Rated 5 de 5 de par Strong Bands Clean and strong bands detected using human cardiac stem cell lysate (30ug protein). Worked at 1:500 primary dilution with 1:1000 anti-mouse secondary.
Date de publication: 2018-02-20
Rated 5 de 5 de par staining image The test indicates the effectiveness of the results of image enhancement. Experiments show that it is an efficient method
Date de publication: 2017-10-19
Rated 5 de 5 de par Strong signals with clean background I got the sample for western blotting using the lysates from rat cultured neurons and mouse brains, and I got strong calpain 2 signals with the clean background. I use PVDF membrane and the antibody is 1:5000 diluted in 3% milk/TBST.
Date de publication: 2017-09-26
Rated 5 de 5 de par Great I got a free sample for test, and it works very well
Date de publication: 2017-09-07
Rated 4 de 5 de par suitable for IF suitable for formaldehyde-fixed breast cancer cells MCF-7 by IF
Date de publication: 2017-08-26
Rated 5 de 5 de par Robust and clean Antibody works pretty well and performed western blot, band is pretty clear and strong.
Date de publication: 2017-05-23
Rated 5 de 5 de par strong bands Used at 1:500 to detect Calpain 2 in U87MG cells,works good,single specific band detected,staining data very strong.
Date de publication: 2017-02-24
Rated 5 de 5 de par Well-rounded antibody for Calpain 2 Excellent antibody for Calpain 2, strong and specific, giving solid bands in Western blot and clean clear staining in IHC. Great choice!
Date de publication: 2017-01-31
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