C2 Antibody (E-7): sc-373809
- C2 Antibody (E-7) is a mouse monoclonal IgG2a κ C2 antibody, cited in 6 publications, provided at 200 µg/ml
- raised against amino acids 204-503 mapping within an internal region of C2 of human origin
- C2 Antibody (E-7) is recommended for detection of C2 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-C2 Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for C2 Antibody (E-7) for WB and IHC(P) applications. These reagents are now offered in bundles with C2 Antibody (E-7) (see ordering information below).
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C2 Antibody (E-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects C2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-C2 antibody (E-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. C2 protein plays a crucial role in the complement system, which is essential for immune defense. C2 protein is involved in the classical pathway of complement activation, where cleavage into C2a and C2b fragments occurs, with C2a being responsible for binding to C3 convertase, thus facilitating pathogen opsonization and promoting inflammation. This process is vital for pathogen clearance and immune response initiation. C2 deficiency is the most prevalent deficiency in the classical complement pathway and is often associated with autoimmune diseases and increased susceptibility to bacterial infections. The N-terminal extracellular domain 1 of the complement C2 receptor inhibitory trispanning (CRIT) specifically binds to C2 and interacts with the C2a fragment, effectively blocking C2 cleavage and preventing classical pathway C3 convertase formation. This regulatory mechanism highlights C2′s importance in maintaining immune homeostasis and preventing excessive inflammation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
C2 Antibody (E-7) References:
- Continual low-level activation of the classical complement pathway. | Manderson, AP., et al. 2001. J Exp Med. 194: 747-56. PMID: 11560991
- Intravascular schistosomes and complement. | Skelly, PJ. 2004. Trends Parasitol. 20: 370-4. PMID: 15246320
- Complement C2 receptor inhibitor trispanning: a novel human complement inhibitory receptor. | Inal, JM., et al. 2005. J Immunol. 174: 356-66. PMID: 15611259
- [Immunologic tests: C2]. | Kitano, E. and Kitamura, H. 2005. Nihon Rinsho. 63 Suppl 7: 59-62. PMID: 16111188
- Variation in factor B (BF) and complement component 2 (C2) genes is associated with age-related macular degeneration. | Gold, B., et al. 2006. Nat Genet. 38: 458-62. PMID: 16518403
- Mannan-binding lectin activates C3 and the alternative complement pathway without involvement of C2. | Selander, B., et al. 2006. J Clin Invest. 116: 1425-34. PMID: 16670774
- Homozygosity for the IgG2 subclass allotype G2M(n) protects against severe infection in hereditary C2 deficiency. | Jönsson, G., et al. 2006. J Immunol. 177: 722-8. PMID: 16785571
- Complement regulatory proteins and autoimmunity. | Song, WC. 2006. Autoimmunity. 39: 403-10. PMID: 16923540
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C2 Antibody (E-7) | sc-373809 | 200 µg/ml | $322.00 | |||
C2 Antibody (E-7): m-IgG Fc BP-HRP Bundle | sc-529570 | 200 µg Ab; 10 µg BP | $361.00 | |||
C2 Antibody (E-7): m-IgGκ BP-HRP Bundle | sc-522508 | 200 µg Ab, 40 µg BP | $361.00 | |||
C2 Antibody (E-7): m-IgG2a BP-HRP Bundle | sc-547332 | 200 µg Ab; 10 µg BP | $361.00 | |||
C2 Antibody (E-7) X | sc-373809 X | 200 µg/0.1 ml | $322.00 | |||
C2 Antibody (E-7) AC | sc-373809 AC | 500 µg/ml, 25% agarose | $424.00 | |||
C2 Antibody (E-7) HRP | sc-373809 HRP | 200 µg/ml | $322.00 | |||
C2 Antibody (E-7) FITC | sc-373809 FITC | 200 µg/ml | $336.00 | |||
C2 Antibody (E-7) PE | sc-373809 PE | 200 µg/ml | $349.00 | |||
C2 Antibody (E-7) Alexa Fluor® 488 | sc-373809 AF488 | 200 µg/ml | $364.00 | |||
C2 Antibody (E-7) Alexa Fluor® 546 | sc-373809 AF546 | 200 µg/ml | $364.00 | |||
C2 Antibody (E-7) Alexa Fluor® 594 | sc-373809 AF594 | 200 µg/ml | $364.00 | |||
C2 Antibody (E-7) Alexa Fluor® 647 | sc-373809 AF647 | 200 µg/ml | $364.00 | |||
C2 Antibody (E-7) Alexa Fluor® 680 | sc-373809 AF680 | 200 µg/ml | $364.00 | |||
C2 Antibody (E-7) Alexa Fluor® 790 | sc-373809 AF790 | 200 µg/ml | $364.00 |