NUAK1/ARK5 Antibody (B-4): sc-271828

NUAK1 Antibody (B-4) is a mouse monoclonal IgG2a antibody that detects NUAK1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NUAK1, also known as omphk1 (omphalocele kinase 1), is a 658 amino acid protein located in both the nucleus and cytoplasm, belonging to the CAMK Ser/Thr protein kinase family and the SNF1 subfamily. anti-NUAK1 antibody (B-4) plays a crucial role in studying various cellular processes, including cell adhesion, regulation of cell ploidy and senescence, as well as cell proliferation and tumor progression. NUAK1′s cellular location enables interactions with key signaling pathways that govern these processes, making NUAK1 essential for maintaining cellular homeostasis and responding to stress signals. Notably, NUAK1 phosphorylates several critical proteins, including Atm, caspase-6, LATS1, MYPT1, and p53, which are involved in DNA damage response and apoptosis. NUAK1 expression in the developing central nervous system and epidermis highlights NUAK1′s significance in developmental biology, while homozygous Nauk1 mutants exhibit omphalocele, a serious condition resulting from improper closure of the body wall, underscoring NUAK1′s vital role in embryonic development. With diverse functions and critical interactions, NUAK1 (B-4) antibody serves as an invaluable tool for researchers investigating the molecular mechanisms underlying cell growth, differentiation, and disease.

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NUAK1/ARK5 Antibody (B-4) References:

1. NUAK1 knockdown suppresses prostate cancer cell epithelial-mesenchymal transition, migration, and invasion through microRNA-30b-5p.  |  Guan, Y., et al. 2018. Int J Clin Exp Pathol. 11: 5694-5704. PMID: 31949655
2. Suppressing the KIF20A/NUAK1/Nrf2/GPX4 signaling pathway induces ferroptosis and enhances the sensitivity of colorectal cancer to oxaliplatin.  |  Yang, C., et al. 2021. Aging (Albany NY). 13: 13515-13534. PMID: 33819186
3. DKK1 promotes NUAK1 transcriptional expression through the activation Akt in hepatocellular carcinoma.  |  Yao, CY., et al. 2023. Cell Biol Int. 47: 383-393. PMID: 36480792
4. NUAK1 governs centrosome replication in pancreatic cancer via MYPT1/PP1β and GSK3β-dependent regulation of PLK4.  |  Whyte, D., et al. 2023. Mol Oncol. 17: 1212-1227. PMID: 36975767
5. NUAK1 promotes tumor metastasis through upregulating slug transcription in esophageal squamous cell carcinoma.  |  Yang, H., et al. 2023. Cancer Cell Int. 23: 258. PMID: 37919754
6. NUAK1 coordinates growth factor-dependent activation of mTORC2 and Akt signaling.  |  Palma, M., et al. 2023. Cell Biosci. 13: 232. PMID: 38135881
7. The AMPK-related kinase NUAK1 controls cortical axons branching by locally modulating mitochondrial metabolic functions.  |  Lanfranchi, M., et al. 2024. Nat Commun. 15: 2487. PMID: 38514619
8. NUAK1 promotes metabolic dysfunction-associated steatohepatitis progression by activating Caspase 6-driven pyroptosis and inflammation.  |  Sheng, M., et al. 2024. Hepatol Commun. 8: PMID: 38967580
9. NUAK1 activates STAT5/GLI1/SOX2 signaling to enhance cancer cell expansion and drives chemoresistance in gastric cancer.  |  Cao, L., et al. 2024. Cell Rep. 43: 114446. PMID: 38996065
10. NUAK1-Mediated Phosphorylation of NADK Mitigates ROS Accumulation to Promote Osimertinib Resistance in Non-Small Cell Lung Carcinoma.  |  Lin, W., et al. 2024. Cancer Res. 84: 4081-4098. PMID: 39159134

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NUAK1/ARK5 Antibody (B-4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-NUAK1/ARK5 Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NUAK1/ARK5 Antibody (B-4) Specifications

Alternate Product Name: ARK5

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 526-661

Epitope Target: NUAK1

Epitope Species: human

Product Reactivity/Detection: NUAK1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for NUAK1/ARK5 Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred NUAK1/ARK5 antibody is NUAK1/ARK5 Antibody (A-9): sc-271827.