Arginase 2/ARG2 Antibody (A-10): sc-393496

Arg2 Antibody (A-10) is a mouse monoclonal IgA kappa light chain antibody that specifically targets an epitope between amino acids 304–335 near the C-terminus of human Arginase 2 (ARG2), also known as Arginase II. ARG2 is a mitochondrial enzyme that catalyzes the hydrolysis of L-arginine into L-ornithine and urea, playing a crucial role in the urea cycle and polyamine biosynthesis. Structurally, ARG2 is a 354 amino acid protein that forms a homotrimer, with each subunit containing a binuclear manganese cluster essential for catalytic activity. The structural configuration of ARG2 is significant because the active site architecture and manganese coordination are critical for substrate binding and enzymatic function. Unlike its cytosolic counterpart Arginase I, which is predominantly expressed in the liver, ARG2 is primarily found in extrahepatic tissues such as the kidney and small intestine. The mitochondrial localization of ARG2 influences nitric oxide production by modulating L-arginine availability, thereby affecting vascular function and immune responses. Dysregulation of ARG2 has been implicated in various pathological conditions, including cardiovascular diseases, neurodegenerative disorders, and cancer. Anti-ARG2 antibody (A-10) demonstrates reactivity with ARG2 of mouse, rat, human, and additional species like canine, bovine, and porcine origin in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). ARG2 monoclonal antibody (A-10) is a valuable tool for researchers studying the structural and functional aspects of ARG2, contributing to a deeper understanding of its role in physiological and pathological processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Arginase 2/ARG2 Antibody (A-10) References:

1. Implications of the S-shaped domain in the quaternary structure of human arginase.  |  Mora, A., et al. 2000. Biochim Biophys Acta. 1476: 181-90. PMID: 10669784
2. Structure and function of arginases.  |  Ash, DE. 2004. J Nutr. 134: 2760S-2764S; discussion 2765S-2767S. PMID: 15465781
3. Reducing arginase activity via dietary manganese deficiency enhances endothelium-dependent vasorelaxation of rat aorta.  |  Ensunsa, JL., et al. 2004. Exp Biol Med (Maywood). 229: 1143-53. PMID: 15564441
4. Arginase activity concentration marking in monitoring of hepatocytes function after orthotopic liver transplantation--preliminary report.  |  Grodzicki, M., et al. 2004. Ann Transplant. 9: 54-7. PMID: 15759549
5. Remission of hepatocellular carcinoma with arginine depletion induced by systemic release of endogenous hepatic arginase due to transhepatic arterial embolisation, augmented by high-dose insulin: arginase as a potential drug candidate for hepatocellular carcinoma.  |  Cheng, PN., et al. 2005. Cancer Lett. 224: 67-80. PMID: 15911102
6. ETNPPL impairs autophagy through regulation of the ARG2-ROS signaling axis, contributing to palmitic acid-induced hepatic insulin resistance.  |  Wang, C., et al. 2023. Free Radic Biol Med. 199: 126-140. PMID: 36841363
7. ARG2 knockdown promotes G0/G1 cell cycle arrest and mitochondrial dysfunction in adenomyosis via regulation NF-κB and Wnt/Β-catenin signaling cascades.  |  Xu, Y., et al. 2024. Int Immunopharmacol. 140: 112817. PMID: 39116499
8. Immunological identity of the two different molecular mass constitutive subunits of liver arginase.  |  Diez, A., et al. 1994. Biol Chem Hoppe Seyler. 375: 537-41. PMID: 7811393
9. Molecular cloning of cDNA for nonhepatic mitochondrial arginase (arginase II) and comparison of its induction with nitric oxide synthase in a murine macrophage-like cell line.  |  Gotoh, T., et al. 1996. FEBS Lett. 395: 119-22. PMID: 8898077
10. Chromosomal localization of the human arginase II gene and tissue distribution of its mRNA.  |  Gotoh, T., et al. 1997. Biochem Biophys Res Commun. 233: 487-91. PMID: 9144563
11. Differential expression of arginase and iNOS in the lung in sepsis.  |  Carraway, MS., et al. 1998. Exp Lung Res. 24: 253-68. PMID: 9635249
12. Hyperargininemia due to liver arginase deficiency.  |  Crombez, EA. and Cederbaum, SD. 2005. Mol Genet Metab. 84: 243-51. PMID: 15694174

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Arginase 2/ARG2 Antibody (A-10) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-Arginase 2/ARG2 Antibody (A-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Arginase 2/ARG2 Antibody (A-10) Specifications

Alternate Product Name: Arg2

Product Clone Name: A-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgA kappa light chain

Amino Acids: 304-335

Epitope Target: Arg2

Epitope Species: human

Product Reactivity/Detection: Arg2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgA kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for Arginase 2/ARG2 Antibody (A-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Arginase 2/ARG2 antibody is Arginase 2/ARG2 Antibody (C-8): sc-271443.