Aldehyde dehydrogenase 1-A1/ALDH1A1 Double Nickase Plasmid (h): sc-400782-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Aldehyde dehydrogenase 1-A1/ALDH1A1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Aldehyde dehydrogenase 1-A1/ALDH1A1 Double Nickase Plasmid (h) and Aldehyde dehydrogenase 1-A1/ALDH1A1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ALDH1A1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Aldehyde dehydrogenase 1-A1/ALDH1A1 Antibody (A-6): sc-398578
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aldehyde dehydrogenase 1-A1/ALDH1A1 Double Nickase Plasmid (h) | sc-400782-NIC | 20 µg | $410.00 | |||
Aldehyde dehydrogenase 1-A1/ALDH1A1 Double Nickase Plasmid (h2) | sc-400782-NIC-2 | 20 µg | $410.00 |
Human ALDH1A1 encodes a cytosolic NAD(P)+-dependent aldehyde dehydrogenase that oxidizes endogenous and xenobiotic aldehydes to their corresponding carboxylic acids, contributing to cellular detoxification and redox homeostasis. It plays a central role in retinoid metabolism by converting retinaldehyde to retinoic acid, linking ALDH1A1 activity to transcriptional programs that influence differentiation and stress responses. ALDH1A1 expression and enzymatic activity are frequently used as functional readouts in studies of epithelial biology and cellular plasticity, and altered regulation has been associated with metabolic adaptation and oxidative stress handling in disease contexts. These properties make ALDH1A1 a widely studied node in pathways controlling aldehyde clearance, retinoic acid signaling, and cellular resilience.
Aldehyde dehydrogenase 1-A1/ALDH1A1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ALDH1A1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ALDH1A1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ALDH1A1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ALDH1A1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.