ZAP-70 Antibody (24A): sc-136126
- ZAP-70 Antibody (24A) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
- raised against amino acids 316-325 of ZAP-70 of human origin
- recommended for detection of ZAP-70 of mouse, rat and human origin by WB and IP
- See ZAP-70 (1E7.2): sc-32760 for ZAP-70 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for ZAP-70 Antibody (24A). This work is in progress.
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ZAP-70 Antibody (24A) is a mouse monoclonal IgG1 light chain antibody that detects ZAP-70 in mouse, rat, and human samples through western blotting (WB) and immunoprecipitation (IP) applications. ZAP-70, a crucial tyrosine kinase, plays a significant role in T cell receptor signaling, which is essential for T lymphocyte activation and immune response. Located primarily in the cytoplasm, ZAP-70 is recruited to the T cell receptor complex upon antigen recognition, where phosphorylation activates downstream signaling pathways that lead to T cell proliferation and differentiation. The importance of ZAP-70 in immune function is underscored by its association with severe combined immunodeficiency when mutated, highlighting a critical role in maintaining effective immune responses. Anti-ZAP-70 antibody (24A) serves as an invaluable tool for researchers studying T cell signaling and its implications in immunology and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ZAP-70 Antibody (24A) References:
- ZAP-70 methylation status is associated with ZAP-70 expression status in chronic lymphocytic leukemia. | Corcoran, M., et al. 2005. Haematologica. 90: 1078-88. PMID: 16079107
- Clinical utility of assessing ZAP-70 and CD38 in chronic lymphocytic leukemia. | Rassenti, LZ. and Kipps, TJ. 2006. Cytometry B Clin Cytom. 70: 209-13. PMID: 16906583
- Distinct roles for Syk and ZAP-70 during early thymocyte development. | Palacios, EH. and Weiss, A. 2007. J Exp Med. 204: 1703-15. PMID: 17606633
- ZAP-70 enhances IgM signaling independent of its kinase activity in chronic lymphocytic leukemia. | Chen, L., et al. 2008. Blood. 111: 2685-92. PMID: 18048647
- Combined analysis of ZAP-70 and CD38 expression in sudanese patients with B-cell chronic lymphocytic leukemia. | Basabaeen, AA., et al. 2019. BMC Res Notes. 12: 282. PMID: 31122288
- Developmental partitioning of SYK and ZAP70 prevents autoimmunity and cancer. | Sadras, T., et al. 2021. Mol Cell. 81: 2094-2111.e9. PMID: 33878293
- Ssu72 phosphatase directly binds to ZAP-70, thereby providing fine-tuning of TCR signaling and preventing spontaneous inflammation. | Ko, JS., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 34452999
- T and NK cell lymphoma cell lines do not rely on ZAP-70 for survival. | de Mel, S., et al. 2022. PLoS One. 17: e0261469. PMID: 35077445
- Novel Compound Heterozygous ZAP70 R37G A507T Mutations in Infant with Severe Immunodeficiency. | Benavides, N., et al. 2023. J Clin Immunol. 44: 27. PMID: 38129328
- Essential role for ZAP-70 in both positive and negative selection of thymocytes. | Negishi, I., et al. 1995. Nature. 376: 435-8. PMID: 7630421
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZAP-70 Antibody (24A) | sc-136126 | 50 µg/0.5 ml | $322.00 |