WDR55 Antibody (A-5): sc-514225
- WDR55 Antibody (A-5) is a mouse monoclonal IgG1 κ WDR55 antibody provided at 200 µg/ml
- raised against amino acids 28-120 mapping near the C-terminus of WDR55 of human origin
- WDR55 Antibody (A-5) is recommended for detection of WDR55 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-WDR55 Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for WDR55 Antibody (A-5) for WB applications. These reagents are now offered in bundles with WDR55 Antibody (A-5) (see ordering information below).
WDR55 Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects WDR55 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-WDR55 antibody (A-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. WDR55, consisting of 383 amino acids, is primarily located in the nucleus and cytoplasm and contains seven WD repeats, which are critical for function. These WD repeats form a characteristic propeller-like structure that facilitates protein-protein interactions, allowing WDR55 to play a significant role in ribosomal RNA synthesis regulation. This function is essential for proper cellular growth and development, particularly during organogenesis, as WDR55 influences ribosome production necessary for protein synthesis. WDR55 is encoded by a gene located on human chromosome 5q31.3 and exists in two alternatively spliced isoforms, highlighting potential for diverse regulatory roles within cells. WDR55′s ability to modulate rRNA synthesis underscores importance in maintaining cellular homeostasis and responding to developmental cues, making WDR55 a valuable target for research in cellular biology and developmental processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
WDR55 Antibody (A-5) References:
- The WD repeat: a common architecture for diverse functions. | Smith, TF., et al. 1999. Trends Biochem Sci. 24: 181-5. PMID: 10322433
- Thirty-plus functional families from a single motif. | Yu, L., et al. 2000. Protein Sci. 9: 2470-6. PMID: 11206068
- WD-repeat proteins: structure characteristics, biological function, and their involvement in human diseases. | Li, D. and Roberts, R. 2001. Cell Mol Life Sci. 58: 2085-97. PMID: 11814058
- The WD-repeat protein superfamily in Arabidopsis: conservation and divergence in structure and function. | van Nocker, S. and Ludwig, P. 2003. BMC Genomics. 4: 50. PMID: 14672542
- Phylogenetic, structural and functional relationships between WD- and Kelch-repeat proteins. | Hudson, AM. and Cooley, L. 2008. Subcell Biochem. 48: 6-19. PMID: 18925367
- The ancient regulatory-protein family of WD-repeat proteins. | Neer, EJ., et al. 1994. Nature. 371: 297-300. PMID: 8090199
- Folding of proteins with WD-repeats: comparison of six members of the WD-repeat superfamily to the G protein beta subunit. | Garcia-Higuera, I., et al. 1996. Biochemistry. 35: 13985-94. PMID: 8909296
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
WDR55 Antibody (A-5) | sc-514225 | 200 µg/ml | $322.00 | |||
WDR55 Antibody (A-5): m-IgG Fc BP-HRP Bundle | sc-531070 | 200 µg Ab; 10 µg BP | $361.00 | |||
WDR55 Antibody (A-5): m-IgGκ BP-HRP Bundle | sc-524654 | 200 µg Ab, 40 µg BP | $361.00 | |||
WDR55 Antibody (A-5) AC | sc-514225 AC | 500 µg/ml, 25% agarose | $424.00 | |||
WDR55 Antibody (A-5) HRP | sc-514225 HRP | 200 µg/ml | $322.00 | |||
WDR55 Antibody (A-5) FITC | sc-514225 FITC | 200 µg/ml | $336.00 | |||
WDR55 Antibody (A-5) PE | sc-514225 PE | 200 µg/ml | $349.00 | |||
WDR55 Antibody (A-5) Alexa Fluor® 488 | sc-514225 AF488 | 200 µg/ml | $364.00 | |||
WDR55 Antibody (A-5) Alexa Fluor® 546 | sc-514225 AF546 | 200 µg/ml | $364.00 | |||
WDR55 Antibody (A-5) Alexa Fluor® 594 | sc-514225 AF594 | 200 µg/ml | $364.00 | |||
WDR55 Antibody (A-5) Alexa Fluor® 647 | sc-514225 AF647 | 200 µg/ml | $364.00 | |||
WDR55 Antibody (A-5) Alexa Fluor® 680 | sc-514225 AF680 | 200 µg/ml | $364.00 | |||
WDR55 Antibody (A-5) Alexa Fluor® 790 | sc-514225 AF790 | 200 µg/ml | $364.00 |