Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TUSC2 CRISPR/Cas9 KO Plasmid (h): sc-407062
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TUSC2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TUSC2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TUSC2 Antibody (1550CT388.74.12): sc-517369
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TUSC2 CRISPR/Cas9 KO Plasmid (h) | sc-407062 | 20 µg | $397.00 |
Overview
TUSC2 (tumor suppressor candidate 2), also known as FUS1, encodes a small mitochondrial-associated protein implicated in the regulation of cellular homeostasis, including control of reactive oxygen species, calcium signaling, and stress-induced apoptosis. TUSC2 has been linked to modulation of kinase-driven pathways such as PI3K/AKT and MAPK signaling, influencing proliferation and survival programs in epithelial cells. Reduced expression or functional loss of TUSC2 is frequently observed in multiple tumor types and is associated with altered mitochondrial function and dysregulated growth control. These properties make TUSC2 a useful target for dissecting mitochondria-to-nucleus signaling, oxidative stress responses, and pathway dependencies relevant to cancer biology.
TUSC2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TUSC2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TUSC2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TUSC2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TUSC2 protein expression.
This CRISPR knockout system enables efficient generation of TUSC2-deficient cell models for investigation of TUSC2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TUSC2 exon(s) critical for TUSC2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TUSC2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TUSC2 CRISPR/Cas9 KO Plasmid (h) and TUSC2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TUSC2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TUSC2 HDR Plasmid (h) and TUSC2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TUSC2 homology arms to support homology-directed repair at defined TUSC2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.