TIGAR Antibody (G-2): sc-74577

TIGAR Antibody (G-2) is a mouse monoclonal IgG2a antibody that detects TIGAR (TP53-induced glycolysis and apoptosis regulator) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TIGAR is a 270 amino acid protein that plays a crucial role in cellular metabolism and stress response by modulating glycolysis and the pentose phosphate pathway. Induced by the p53 tumor suppressor pathway, TIGAR helps protect cells from oxidative stress by lowering intracellular levels of fructose-2,6-bisphosphate, which in turn inhibits glycolysis and redirects metabolic flux towards the pentose phosphate shunt. This shift is vital as TIGAR not only reduces the production of reactive oxygen species, which can cause DNA damage and trigger apoptosis, but also generates NADPH, a critical cofactor for biosynthetic reactions and antioxidant defense. TIGAR′s ability to regulate these metabolic pathways underscores TIGAR′s importance in cellular survival and potential implications in cancer biology, where p53 mutations can lead to dysregulated metabolism and increased oxidative stress. Anti-TIGAR antibody (G-2) serves as an essential tool for researchers investigating the intricate balance between metabolism and cell survival in various physiological and pathological contexts.

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TIGAR Antibody (G-2) References:

1. A TIGAR-regulated metabolic pathway is critical for protection of brain ischemia.  |  Li, M., et al. 2014. J Neurosci. 34: 7458-71. PMID: 24872551
2. TIGAR promotes neural stem cell differentiation through acetyl-CoA-mediated histone acetylation.  |  Zhou, W., et al. 2019. Cell Death Dis. 10: 198. PMID: 30814486
3. TIGAR alleviates ischemia/reperfusion-induced autophagy and ischemic brain injury.  |  Zhang, DM., et al. 2019. Free Radic Biol Med. 137: 13-23. PMID: 30978385
4. Dynamic ROS Control by TIGAR Regulates the Initiation and Progression of Pancreatic Cancer.  |  Cheung, EC., et al. 2020. Cancer Cell. 37: 168-182.e4. PMID: 31983610
5. Regulatory role of TIGAR on endothelial metabolism and angiogenesis.  |  He, X., et al. 2021. J Cell Physiol. 236: 7578-7590. PMID: 33928637
6. Knockout of TIGAR enhances myocardial phosphofructokinase activity and preserves diastolic function in heart failure.  |  He, X., et al. 2022. J Cell Physiol. 237: 3317-3327. PMID: 35621078
7. The role of TIGAR in nervous system diseases.  |  Huang, B., et al. 2022. Front Aging Neurosci. 14: 1023161. PMID: 36437984
8. TIGAR Protects Cochlear Hair Cells against Teicoplanin-Induced Damage.  |  Zhang, Q., et al. 2023. Mol Neurobiol. 60: 3788-3802. PMID: 36943624
9. TIGAR Deficiency Blunts Angiotensin-II-Induced Cardiac Hypertrophy in Mice.  |  He, X., et al. 2024. Int J Mol Sci. 25: PMID: 38397106
10. TIGAR relieves PCOS by inhibiting granulosa cell apoptosis and oxidative stress through activating Nrf2.  |  Li, Y., et al. 2024. Mol Cell Endocrinol. 594: 112381. PMID: 39341450

The preferred TIGAR antibody is TIGAR Antibody (E-2): sc-166290.