SSEA-4 Antibody (813-70): sc-21704

SSEA-4 Antibody (813-70) is a mouse monoclonal IgG3 kappa light chain antibody that detects SSEA-4 protein of mouse, rat, and human origin by immunofluorescence (IF) and flow cytometry (FCM). SSEA-4 (813-70) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SSEA-4 plays a crucial role in identifying and characterizing pluripotent stem cells, serving as a key marker for undifferentiated embryonic stem cells and embryonal carcinoma cells. SSEA-4 (813-70) monoclonal antibody recognizes surface-localized SSEA-4, which helps maintain stem cells in an undifferentiated state, enabling indefinite proliferation while preserving differentiation potential. Anti-SSEA-4 antibody (813-70) aids in isolating and studying stem cells by distinguishing undifferentiated from differentiated cells, supporting research in developmental biology and regenerative medicine. SSEA-4 contributes to cell-cell interactions and signaling pathways essential for stem cell maintenance and differentiation, making SSEA-4 (813-70) monoclonal antibody valuable for understanding stem cell biology and therapeutic applications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SSEA-4 Antibody (813-70) References:

1. Surface antigens of human embryonic stem cells: changes upon differentiation in culture.  |  Draper, JS., et al. 2002. J Anat. 200: 249-58. PMID: 12033729
2. Human embryonal carcinoma cells and their differentiation in culture.  |  Andrews, PW., et al. 1987. Int J Androl. 10: 95-104. PMID: 2438234
3. mTORC1 maintains the tumorigenicity of SSEA-4(+) high-grade osteosarcoma.  |  Zhang, W., et al. 2015. Sci Rep. 5: 9604. PMID: 25853231
4. The role of Globo H and SSEA-4 in the development and progression of cancer, and their potential as therapeutic targets.  |  Sigal, DS., et al. 2022. Future Oncol. 18: 117-134. PMID: 34734786
5. SSEA-4 positive dental pulp stem cells from deciduous teeth and their induction to neural precursor cells.  |  Lopez-Lozano, AP., et al. 2022. Head Face Med. 18: 9. PMID: 35236383
6. Targeting Stage-Specific Embryonic Antigen 4 (SSEA-4) in Triple Negative Breast Cancer by CAR T Cells Results in Unexpected on Target/off Tumor Toxicities in Mice.  |  Pfeifer, R., et al. 2023. Int J Mol Sci. 24: PMID: 37298141
7. Efficacy and Safety of Glycosphingolipid SSEA-4 Targeting CAR-T Cells in an Ovarian Carcinoma Model.  |  Monzo, HJ., et al. 2023. Mol Cancer Ther. 22: 1319-1331. PMID: 37486980
8. Increased expression of SSEA-4 on TKI-resistant non-small cell lung cancer with EGFR-T790M mutation.  |  Chen, NY., et al. 2024. Proc Natl Acad Sci U S A. 121: e2313397121. PMID: 38252815
9. Development of Subcutaneous SSEA3- or SSEA4-Positive Cell Capture Device.  |  Nakayama, Y. and Iwai, R. 2024. Bioengineering (Basel). 11: PMID: 38927821
10. Isolation of a primate embryonic stem cell line.  |  Thomson, JA., et al. 1995. Proc Natl Acad Sci U S A. 92: 7844-8. PMID: 7544005
11. Pluripotent cell lines derived from common marmoset (Callithrix jacchus) blastocysts.  |  Thomson, JA., et al. 1996. Biol Reprod. 55: 254-9. PMID: 8828827
12. Derivation of pluripotent stem cells from cultured human primordial germ cells.  |  Shamblott, MJ., et al. 1998. Proc Natl Acad Sci U S A. 95: 13726-31. PMID: 9811868

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SSEA-4 Antibody (813-70) has 222 citations in a variety of scientific publications.

Primary antibodies like Anti-SSEA-4 Antibody (813-70) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SSEA-4 Antibody (813-70) Specifications

Alternate Product Name: Stage-specific embryonic antigen 4 (SSEA4)

Product Clone Name: 813-70

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids:

Epitope Target: SSEA-4

Epitope Species: human

Product Reactivity/Detection: SSEA-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IF, FCM

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 222 citations in various scientific publications

Available Conjugates/Forms for SSEA-4 Antibody (813-70): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin