PRK2 Antibody (A-3): sc-271971
- PRK2 Antibody (A-3) is a mouse monoclonal IgG2a κ PRK2 antibody, cited in 4 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 949-978 at the C-terminus of PRK2 of human origin
- PRK2 Antibody (A-3) is recommended for detection of PRK2 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-PRK2 Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PRK2 Antibody (A-3) for WB and IHC(P) applications. These reagents are now offered in bundles with PRK2 Antibody (A-3) (see ordering information below).
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PRK2 Antibody (A-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects PRK2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PRK2 (A-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PRK2, also known as protein kinase C-related kinase 2, plays a crucial role in cellular signaling pathways, particularly in regulating actin dynamics and cytoskeletal organization. PRK2 is primarily located in the cytoplasm and is involved in modulating various cellular processes, including cell migration, proliferation, and apoptosis. Proper localization of PRK2 is essential for function, as PRK2 interacts with Rho GTPases to influence actin filament organization and stability, which are vital for maintaining cell shape and facilitating movement. Additionally, PRK2 undergoes post-translational modifications, such as phosphorylation, which can alter activity and interactions with other proteins, further emphasizing importance in cellular signaling networks.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PRK2 Antibody (A-3) References:
- A novel serine/threonine kinase binding the Ras-related RhoA GTPase which translocates the kinase to peripheral membranes. | Leung, T., et al. 1995. J Biol Chem. 270: 29051-4. PMID: 7493923
- Purification and characterization of a fatty acid-activated protein kinase (PKN) from rat testis. | Kitagawa, M., et al. 1995. Biochem J. 310 (Pt 2): 657-64. PMID: 7654208
- Protein kinase N (PKN) and PKN-related protein rhophilin as targets of small GTPase Rho. | Watanabe, G., et al. 1996. Science. 271: 645-8. PMID: 8571126
- Identification of a putative target for Rho as the serine-threonine kinase protein kinase N. | Amano, M., et al. 1996. Science. 271: 648-50. PMID: 8571127
- The role of the unique motifs in the amino-terminal region of PKN on its enzymatic activity. | Kitagawa, M., et al. 1996. Biochem Biophys Res Commun. 220: 963-8. PMID: 8607876
- The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase. | Ishizaki, T., et al. 1996. EMBO J. 15: 1885-93. PMID: 8617235
- PKN associates and phosphorylates the head-rod domain of neurofilament protein. | Mukai, H., et al. 1996. J Biol Chem. 271: 9816-22. PMID: 8621664
- Characterization of the interaction between RhoA and the amino-terminal region of PKN. | Shibata, H., et al. 1996. FEBS Lett. 385: 221-4. PMID: 8647255
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRK2 Antibody (A-3) | sc-271971 | 200 µg/ml | $322.00 | |||
PRK2 Antibody (A-3): m-IgG Fc BP-HRP Bundle | sc-529294 | 200 µg Ab; 10 µg BP | $361.00 | |||
PRK2 Antibody (A-3): m-IgGκ BP-HRP Bundle | sc-522111 | 200 µg Ab, 40 µg BP | $361.00 | |||
PRK2 Antibody (A-3): m-IgG2a BP-HRP Bundle | sc-547296 | 200 µg Ab; 10 µg BP | $361.00 | |||
PRK2 Antibody (A-3) AC | sc-271971 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PRK2 Antibody (A-3) HRP | sc-271971 HRP | 200 µg/ml | $322.00 | |||
PRK2 Antibody (A-3) FITC | sc-271971 FITC | 200 µg/ml | $336.00 | |||
PRK2 Antibody (A-3) PE | sc-271971 PE | 200 µg/ml | $349.00 | |||
PRK2 Antibody (A-3) Alexa Fluor® 488 | sc-271971 AF488 | 200 µg/ml | $364.00 | |||
PRK2 Antibody (A-3) Alexa Fluor® 546 | sc-271971 AF546 | 200 µg/ml | $364.00 | |||
PRK2 Antibody (A-3) Alexa Fluor® 594 | sc-271971 AF594 | 200 µg/ml | $364.00 | |||
PRK2 Antibody (A-3) Alexa Fluor® 647 | sc-271971 AF647 | 200 µg/ml | $364.00 | |||
PRK2 Antibody (A-3) Alexa Fluor® 680 | sc-271971 AF680 | 200 µg/ml | $364.00 | |||
PRK2 Antibody (A-3) Alexa Fluor® 790 | sc-271971 AF790 | 200 µg/ml | $364.00 | |||
PRK2 (A-3) Neutralizing Peptide | sc-271971 P | 100 µg/0.5 ml | $69.00 |