Polycystin-1 Anticuerpo (7E12) recomendado para detectarPolycystin-1 de mouse, rat y human origen, mediante WB, IP, IF y ELISA

Polycystin-1 Anticuerpo (7E12): sc-130554

Polycystin-1 Anticuerpo (7E12) clasificado 4.8 de 5 por 6.
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Ficha técnica

    • Anti-Polycystin-1 Anticuerpo 7E12 es un monoclonal de ratón IgG1 (kappa light chain) Polycystin-1 Anticuerpo proporcionado como 200 µg/ml
    • raised against amino acids 24-180 corresponding to the N-terminal leucine rich domain of Polycystin-1 of human origin
    • Polycystin-1 Anticuerpo (7E12) es recomendado para detectar Polycystin-1 de mouse, rat y human origen, mediante WB, IP, IF y ELISA
    • Polycystin-1 Anticuerpo (7E12) es disponible conjugado a agarosa para IP; y a Ficoeritrina o fluorosceina para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Ver m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nuestras altamente recomendadas alternativa a los anticuerpos secundarios convencionales, anti-IgG de ratón.
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de Polycystin-1 (7E12): sc-130554.

What's the gel percentage used for this polycystin-1 antibody in WB? Also, is there any suitable reference protein or markers?

Preguntado por: yybio
Thank you for your question. We used a 4-6% gel for this very large protein. These will be very fragile, so handle carefully. Please contact our Technical Service Department for suggestions of suitable high molecular weight proteins that could be used as a reference or molecular weight marker.
Respondida por: Technical Support
Fecha de publicación: 2019-08-17

Can you provide with a FACS protocol for this antibody (polycystin-1, 7E12) on cell lines?

Preguntado por: Curious2
Thank you for your question. We do not have a specific FACS protocol for use with this antibody. Please check our general protocols online, or contact our Technical Support for more information.
Respondida por: Technical Support
Fecha de publicación: 2019-04-13

I recently ordered this mAb conjugated to PE. I'm attempting to use this mAb to quantitate a cell surface antigen - to do that I need to know the ratio of PE molecules per mAb. I.E., do you know if there is one PE bound to each mAb, or two, etc?

Preguntado por: Jeff R
Thank you for your question. For PE conjugated antibodies, the ratio is 1:1, so there is 1 PE per antibody molecule.
Respondida por: Tech Service
Fecha de publicación: 2019-03-25

Which part does this antibody (polycystin-1, 7E12) recognize?

Preguntado por: yybio
Thank you for your question. This monoclonal antibody was raised against amino acids 24-180 corresponding to the N-terminal leucine rich domain of Polycystin-1 of human origin. We will update our product description for future reference.
Respondida por: Technical Support
Fecha de publicación: 2018-12-10

Is it possible to use the AC conjugated Polycystin-1 Antibody (7E12): sc-130554 AC in both Western Blot and IP?

Preguntado por: jenni
Thank you for your inquiry. We do not recommend the use of the AC conjugated form in Western blot. The AC conjugated form of the antibody is specifically used for IP. The unconjugated form of the antibody is the best choice when working in multiple applications.
Respondida por: Technical Support 3
Fecha de publicación: 2017-01-09
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Rated 5 de 5 por de Good for your Western blot! An excellent detection reagent for your WB. If you need to visualize polycystin-1 in human samples, this antibody can do it. Provides a nice clear band and has been cited over ten times.
Fecha de publicación: 2017-01-16
Rated 4 de 5 por de Strong western Very nice western blot data; strong signal with low background
Fecha de publicación: 2017-01-06
Rated 5 de 5 por de Used this antibody in WB and got good results Used this antibody in WB and got good results.
Fecha de publicación: 2015-08-17
Rated 5 de 5 por de Successful application of this antibody in Successful application of this antibody in WB using human PDL fibroblasts -SCBT Publication Review
Fecha de publicación: 2015-04-19
Rated 5 de 5 por de WB publishable data with mIMCD cells WB publishable data with mIMCD cells - SCBT Publication Review
Fecha de publicación: 2015-03-30
Rated 5 de 5 por de This antibody was used in a publication for This antibody was used in a publication for WB on human HEK293 cells - SCBT Publication Review
Fecha de publicación: 2015-03-18
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