PGK1/2 Antibody (A-5): sc-48342

PGK1/2 Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects PGK1/2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PGK1/2 (A-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Phosphoglycerate kinases 1 and 2 (PGK1/2) are crucial glycolytic enzymes that play a significant role in cellular energy metabolism by catalyzing the transfer of a phosphoryl group from 1,3-bisphosphoglycerate to adenosine diphosphate (ADP), resulting in the production of adenosine triphosphate (ATP) and 3-phosphoglycerate. PGK1 is secreted by tumor cells and is involved in angiogenesis, the process of new blood vessel formation, which is vital for tumor growth and metastasis. PGK1 acts as a disulfide reductase, facilitating the reduction of disulfide bonds in the serine proteinase plasmin, which triggers the release of angiostatin, a potent inhibitor of tumor blood vessel formation. The human PGK gene, located on the X chromosome, spans 23 kilobases and is interrupted by 10 introns, with mutations in this region linked to various genetic disorders, including prostate cancer and androgen insensitivity.

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PGK1/2 Antibody (A-5) References:

1. A hemizygous short tandem repeat polymorphism 3' to the human phosphoglycerate kinase gene.  |  Riley, DE., et al. 1999. Mol Biol Rep. 26: 159-65. PMID: 10532310
2. Involvement of a cellular glycolytic enzyme, phosphoglycerate kinase, in Sendai virus transcription.  |  Ogino, T., et al. 1999. J Biol Chem. 274: 35999-6008. PMID: 10585490
3. Phosphoglycerate kinase acts in tumour angiogenesis as a disulphide reductase.  |  Lay, AJ., et al. 2000. Nature. 408: 869-73. PMID: 11130727
4. Overexpression of human phosphoglycerate kinase 1 (PGK1) induces a multidrug resistance phenotype.  |  Duan, Z., et al. 2002. Anticancer Res. 22: 1933-41. PMID: 12174867
5. Novel role of 3-phosphoglycerate kinase, a glycolytic enzyme, in the activation of L-nucleoside analogs, a new class of anticancer and antiviral agents.  |  Krishnan, P., et al. 2003. J Biol Chem. 278: 36726-32. PMID: 12869554
6. Secretion of phosphoglycerate kinase from tumour cells is controlled by oxygen-sensing hydroxylases.  |  Daly, EB., et al. 2004. Biochim Biophys Acta. 1691: 17-22. PMID: 15053920
7. Structure of the human phosphoglycerate kinase gene and the intron-mediated evolution and dispersal of the nucleotide-binding domain.  |  Michelson, AM., et al. 1985. Proc Natl Acad Sci U S A. 82: 6965-9. PMID: 2995995

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PGK1/2 Antibody (A-5) has 18 citations in a variety of scientific publications.

Primary antibodies like Anti-PGK1/2 Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PGK1/2 Antibody (A-5) Specifications

Alternate Product Name: Phosphoglycerate Kinase

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 119-418

Epitope Target: PGK1

Epitope Species: human

Product Reactivity/Detection: PGK1 and PGK2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 18 citations in various scientific publications

Available Conjugates/Forms for PGK1/2 Antibody (A-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin