PBEF Anticuerpo (E-3) recomendado para detectarPBEF de mouse, rat y human origen, mediante WB, IP, IF y ELISA

PBEF Anticuerpo (E-3): sc-393444

PBEF Anticuerpo (E-3) clasificado 5.0 de 5 por 13.
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Ficha técnica

    • Anti-PBEF Anticuerpo E-3 es un monoclonal de ratón IgG2b κ PBEF Anticuerpo, ver las 2 publicaciones, proporcionado como 200 µg/ml
    • producido contra los amino ácidos 1-300 localizados en el N-terminus de PBEF de origen human
    • PBEF Anticuerpo (E-3) es recomendado para detectar PBEF de mouse, rat y human origen, mediante WB, IP, IF y ELISA
    • PBEF Anticuerpo (E-3) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de PBEF (E-3): sc-393444.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for PBEF Antibody (E-3) for WB applications. This reagent is now offered in a bundle with PBEF Antibody (E-3) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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What is the recommended fixation method for immunofluorescence staining with PBEF (E-3): sc-393444 monoclonal antibody?

Preguntado por: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Respondida por: Technical Support
Fecha de publicación: 2017-02-25
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Rated 5 de 5 por de Very good antibody I used this antibody in mouse liver and muscle samples. This antibody works well in both tissues. I was able to get clean bands in WB with 1:500 dilution (by both fluorescence and chemiluminescence).
Fecha de publicación: 2020-07-27
Rated 5 de 5 por de Works well Probed at 1:500 overnight, detected strong bands at the right size
Fecha de publicación: 2017-11-15
Rated 5 de 5 por de Satisfied with the antibody PBEF antibody provides great staining in IF and IHC applications, binding is both strong and specific.The antibody could be performed under isothermal conditions, its specificity was good and the sensitivity.
Fecha de publicación: 2017-10-09
Rated 5 de 5 por de This antibody is very good. PBEF antibody works well for WB at the concentration of 1:200, with satisfied bands.
Fecha de publicación: 2017-05-23
Rated 5 de 5 por de Excellent for WB it works excellent for WB in our lab, will keep usinig it
Fecha de publicación: 2017-05-08
Rated 5 de 5 por de Excellent antibody! I apply for the sample sack of this antibody,the band is special and clear.
Fecha de publicación: 2017-03-29
Rated 5 de 5 por de The Product is Good We tried it detect this sample from dilution 1:800. The researchers confirmed that PBEF Antibody accurately detects rat with high sensitivity and specificity. We were convinced the antibody.
Fecha de publicación: 2017-02-13
Rated 5 de 5 por de WB and worked fine This gave very strong signal in our Western, we use dilution to 1:400 and it worked well.
Fecha de publicación: 2017-02-12
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