p-Trk Antibody (E-6): sc-8058

p-Trk Antibody (E-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects Trk phosphorylated at Tyr 496, along with correspondingly phosphorylated Trk B and Trk C, in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). p-Trk (E-6) antibody is available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. The Trk proto-oncogene encodes a membrane-spanning protein tyrosine kinase, Trk gp140, which is crucial for neuronal development and function, as expression is primarily restricted to neurons originating from the sensory spinal and cranial ganglia. The phosphorylation of Trk A by nerve growth factor (NGF) is a rapid and specific process that occurs even at picomolar concentrations, highlighting the physiological significance of NGF in neuronal signaling. This phosphorylation is essential for mediating the survival, differentiation, and growth of neurons, making Trk A a vital component in neurotrophic signaling pathways. In contrast, Trk B is phosphorylated by brain-derived neurotrophic factor (BDNF), while Trk C phosphorylation is induced by neurotrophin-3 (NT-3), illustrating the diverse roles of these neurotrophins in neuronal health and development.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p-Trk Antibody (E-6) References:

1. The trk tyrosine protein kinase mediates the mitogenic properties of nerve growth factor and neurotrophin-3.  |  Cordon-Cardo, C., et al. 1991. Cell. 66: 173-83. PMID: 1649007
2. The trkB tyrosine protein kinase is a receptor for brain-derived neurotrophic factor and neurotrophin-3.  |  Klein, R., et al. 1991. Cell. 66: 395-403. PMID: 1649702
3. Tyrosine phosphorylation and tyrosine kinase activity of the trk proto-oncogene product induced by NGF.  |  Kaplan, DR., et al. 1991. Nature. 350: 158-60. PMID: 1706478
4. The trk family of tyrosine protein kinase receptors.  |  Barbacid, M., et al. 1991. Biochim Biophys Acta. 1072: 115-27. PMID: 1751544
5. The trk proto-oncogene encodes a receptor for nerve growth factor.  |  Klein, R., et al. 1991. Cell. 65: 189-97. PMID: 1849459
6. High-affinity NGF binding requires coexpression of the trk proto-oncogene and the low-affinity NGF receptor.  |  Hempstead, BL., et al. 1991. Nature. 350: 678-83. PMID: 1850821
7. Expression of the tyrosine kinase receptor gene trkB is confined to the murine embryonic and adult nervous system.  |  Klein, R., et al. 1990. Development. 109: 845-50. PMID: 2171894
8. trkB, a novel tyrosine protein kinase receptor expressed during mouse neural development.  |  Klein, R., et al. 1989. EMBO J. 8: 3701-9. PMID: 2555172
9. Inhibiting TRK Proteins in Clinical Cancer Therapy.  |  Lange, AM. and Lo, HW. 2018. Cancers (Basel). 10: PMID: 29617282
10. Constitutive phosphorylation of TrkC receptors in cultured cerebellar granule neurons might be responsible for the inability of NT-3 to increase neuronal survival and to activate p21 Ras.  |  Zirrgiebel, U. and Lindholm, D. 1996. Neurochem Res. 21: 851-9. PMID: 8873090

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Trk Antibody (E-6) has 62 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Trk Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Trk Antibody (E-6) Specifications

Alternate Product Name: Pan Trk

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Tyr 496

Epitope Target: Trk A

Epitope Species: human

Product Reactivity/Detection: Trk A phosphorylated at Tyr 496 and correspondingly phosphorylated Trk B and Trk C

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 62 citations in various scientific publications

Available Conjugates/Forms for p-Trk Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin