p-Rb Antibody (E-10): sc-271930

p-Rb Antibody (E-10) is a mouse monoclonal IgG3 kappa light chain antibody that detects p-Rb, specifically the dually phosphorylated forms at Thr 821 and Thr 826, in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). p-Rb (E-10) antibody is offered in both non-conjugated and various conjugated formats, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Retinoblastoma protein (Rb) plays a crucial role in regulating the cell cycle, particularly at the G1-S transition, by acting as a tumor suppressor. Rb function is vital for maintaining cellular homeostasis, preventing excessive cell proliferation and promoting differentiation and apoptosis. Phosphorylation of Rb, particularly at Ser 795, leads to inactivation, allowing cell cycle progression. This phosphorylation is part of a complex regulatory mechanism involving multiple phosphorylation sites, including Ser 249/Thr 252, Thr 373, Thr 356, Ser 780, Ser 807/Ser 811, and Thr 821/Thr 826, which collectively modulate Rb activity. Hypophosphorylated Rb represses transcription of cell cycle-related genes through direct protein-protein interactions and recruitment of histone deacetylases, underscoring Rb importance in tumor suppression and oncogenesis prevention, making anti-p-Rb antibody (E-10) an essential tool for researchers studying cancer biology and cell cycle regulation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p-Rb Antibody (E-10) References:

1. Stimulation of DNA polymerase alpha activity by cdk2-phosphorylated Rb protein.  |  Takemura, M., et al. 2001. Biochem Biophys Res Commun. 282: 984-90. PMID: 11352649
2. Relationship between the RB1 mRNA level and the expression of phosphorylated RB protein in human breast cancers: their relevance in cell proliferation activity and patient clinical outcome.  |  Derenzini, M., et al. 2007. Histol Histopathol. 22: 505-13. PMID: 17330805
3. Arterial territory-specific phosphorylated retinoblastoma protein species and CDK2 promote differences in the vascular smooth muscle cell response to mitogens.  |  Lange, M., et al. 2014. Cell Cycle. 13: 315-23. PMID: 24240190
4. OTX2 is a therapeutic target for retinoblastoma and may function as a common factor between C-MYC, CRX, and phosphorylated RB pathways.  |  Li, J., et al. 2015. Int J Oncol. 47: 1703-10. PMID: 26397460
5. The Prognostic Value of the Immunohistochemical Expression of Phosphorylated RB and p16 Proteins in Association with Cyclin D1 and the p53 Pathway in a Large Cohort of Patients with Breast Cancer Treated with Taxane-based Adjuvant Chemotherapy.  |  Gavressea, T., et al. 2017. Anticancer Res. 37: 2947-2957. PMID: 28551632
6. Cell Cycle Protein Expression in Neuroendocrine Tumors: Association of CDK4/CDK6, CCND1, and Phosphorylated Retinoblastoma Protein With Proliferative Index.  |  Shi, Y., et al. 2017. Pancreas. 46: 1347-1353. PMID: 28991877
7. Sertad1 Induces Neurological Injury after Ischemic Stroke via the CDK4/p-Rb Pathway.  |  Li, J., et al. 2022. Mol Cells. 45: 216-230. PMID: 35014620
8. Artesunate and cisplatin synergistically inhibit HNSCC cell growth and promote apoptosis with artesunate‑induced decreases in Rb and phosphorylated Rb levels.  |  Okamoto, H., et al. 2023. Oncol Rep. 50: PMID: 37350399
9. Cytomegalovirus infection induces high levels of cyclins, phosphorylated Rb, and p53, leading to cell cycle arrest.  |  Jault, FM., et al. 1995. J Virol. 69: 6697-704. PMID: 7474079
10. Expression of differentially phosphorylated Rb and mutant p53 proteins in myeloid leukemia cell lines.  |  Sen, S., et al. 1993. Leuk Res. 17: 639-47. PMID: 8355507

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Rb Antibody (E-10) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Rb Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Rb Antibody (E-10) Specifications

Alternate Product Name: RB1

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: Thr 821/826

Epitope Target: Rb

Epitope Species: human

Product Reactivity/Detection: Thr 821 and Thr 826 dually phosphorylated Rb

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for p-Rb Antibody (E-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Rb antibody is Rb Antibody (IF8): sc-102.