p-Rb Antibody (B-7): sc-377527

p-Rb Antibody (B-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects Thr 356 phosphorylated Rb of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). p-Rb (B-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The retinoblastoma protein (Rb) plays a crucial role in regulating the cell cycle, particularly at the G1-S transition, by acting as a transcriptional repressor. This regulation is vital for maintaining proper cell growth and preventing uncontrolled proliferation, which can lead to cancer. Rb achieves this by binding to E2F transcription factors, inhibiting their activity and thereby preventing the expression of genes required for S phase entry. Phosphorylation of Rb at Thr 356 is a key event that leads to inactivation, allowing the cell cycle to progress. Mutations in the Rb gene are implicated in pediatric retinoblastoma and other malignancies, highlighting Rb′s importance in tumor suppression and cell cycle control. anti-Rb antibody (B-7) enables researchers to gain insights into Rb phosphorylation status and function in various cellular processes, making this tool essential for studying cancer biology and cell cycle regulation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Rb Antibody (B-7) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Rb Antibody (B-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Rb Antibody (B-7) Specifications

Alternate Product Name: RB1

Product Clone Name: B-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: Thr 356

Epitope Target: Rb

Epitope Species: human

Product Reactivity/Detection: Thr 356 phosphorylated Rb

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for p-Rb Antibody (B-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Rb antibody is Rb Antibody (IF8): sc-102.