p-c-Fos Antibody (34E4): sc-81485

p-c-Fos Antibody (34E4) is a mouse monoclonal IgG1 kappa light chain antibody raised against phosphorylated human c-Fos, a crucial transcription factor whose activity is regulated by phosphorylation. Anti-p-c-Fos antibody (34E4) recognizes c-Fos only when phosphorylated and demonstrates species cross-reactivity with human and canine samples through Western blotting (WB) and immunoprecipitation (IP) applications. c-Fos monoclonal antibody (34E4) is available in non-conjugated form, enabling researchers to study this critical post-translational modification. As a rapidly induced transcription factor, c-Fos undergoes sophisticated regulation through phosphorylation in response to various stimuli, including growth factors and stress signals. This phosphorylation-dependent activation proves essential for c-Fos′s ability to form heterodimers with Jun family proteins, creating the AP-1 transcription factor complex. The formation of these complexes enables sequence-specific DNA binding, allowing c-Fos to regulate genes involved in cell proliferation, differentiation, and survival. The dynamic nature of c-Fos phosphorylation serves as a molecular switch controlling transcriptional responses to cellular signals, particularly in contexts of inflammation and apoptosis. When this regulatory mechanism becomes dysregulated, the resulting aberrant c-Fos activity can contribute to oncogenic pathways, making c-Fos an important target for cancer research. Through these phosphorylation-dependent functions, p-c-Fos (34E4) antibody provides researchers with a valuable tool for investigating both normal signaling mechanisms and disease pathways.

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p-c-Fos Antibody (34E4) References:

1. Protein kinase D2 potentiates MEK/ERK/RSK signaling, c-Fos accumulation and DNA synthesis induced by bombesin in Swiss 3T3 cells.  |  Sinnett-Smith, J., et al. 2007. J Cell Physiol. 211: 781-90. PMID: 17226786
2. Immunohistochemical analysis of p53, cyclinD1, RB1, c-fos and N-ras gene expression in hepatocellular carcinoma in Iran.  |  Moghaddam, SJ., et al. 2007. World J Gastroenterol. 13: 588-93. PMID: 17278226
3. Cyclic adenosine monophosphate suppresses the transcription of proinflammatory cytokines via the phosphorylated c-Fos protein.  |  Koga, K., et al. 2009. Immunity. 30: 372-83. PMID: 19285436
4. Isolation and characterization of fra-2, an additional member of the fos gene family.  |  Nishina, H., et al. 1990. Proc Natl Acad Sci U S A. 87: 3619-23. PMID: 2110368
5. The product of a fos-related gene, fra-1, binds cooperatively to the AP-1 site with Jun: transcription factor AP-1 is comprised of multiple protein complexes.  |  Cohen, DR., et al. 1989. Genes Dev. 3: 173-84. PMID: 2497053
6. An avian transforming retrovirus isolated from a nephroblastoma that carries the fos gene as the oncogene.  |  Nishizawa, M., et al. 1987. J Virol. 61: 3733-40. PMID: 2824811
7. Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1.  |  Bohmann, D., et al. 1987. Science. 238: 1386-92. PMID: 2825349
8. Chromatin association and DNA binding properties of the c-fos proto-oncogene product.  |  Renz, M., et al. 1987. Nucleic Acids Res. 15: 277-92. PMID: 3103098
9. The Fos protein complex is associated with DNA in isolated nuclei and binds to DNA cellulose.  |  Sambucetti, LC. and Curran, T. 1986. Science. 234: 1417-9. PMID: 3491427
10. Virus induction of osteosarcomas in mice.  |  Finkel, MP., et al. 1966. Science. 151: 698-701. PMID: 5218049

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-c-Fos Antibody (34E4) has 35 citations in a variety of scientific publications.

Primary antibodies like Anti-p-c-Fos Antibody (34E4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-c-Fos Antibody (34E4) Specifications

Alternate Product Name: c-Fos

Product Clone Name: 34E4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 374

Epitope Target: c-Fos

Epitope Species: human

Product Reactivity/Detection: Ser 374 phosphorylated c-Fos

Species Reactivity/Detection: human, canine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 50 µg IgG₁ kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide, 0.1% gelatin, PEG and sucrose

Research Citations: 35 citations in various scientific publications

Available Conjugates/Forms for p-c-Fos Antibody (34E4): N/A

The preferred c-Fos antibody is c-Fos Antibody (D-1): sc-8047.