c-Fos Anticuerpo (D-1) recomendado para detectarc-Fos de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA

c-Fos Anticuerpo (D-1): sc-8047

c-Fos Anticuerpo (D-1) clasificado 3.9 de 5 por 18.
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Ficha técnica

    • c-Fos Anticuerpo (D-1) es un monoclonal de ratón IgG2b (kappa light chain) proporcionado como 200 µg/ml
    • Producido contra los amino ácidos 210-335 en el C-terminus de c-Fos de origen human
    • c-Fos Anticuerpo (D-1) es recomendado para detectar c-Fos de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA
    • c-Fos Anticuerpo (D-1) es disponible conjugado a Alexa Fluor® 546 o Alexa Fluor® 594 para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • TransCruz reactivo para Gel Supershift y ChIP (sc-8047 X, 200 µg/0.1 ml)
    • Ver m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nuestras altamente recomendadas alternativa a los anticuerpos secundarios convencionales, anti-IgG de ratón.
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de c-Fos (D-1): sc-8047.

what is the best replacement for Sc-52

Preguntado por: ismaila
Thank you for your question. This is one of the most popular replacement antibodies for that now discontinued polyclonal antibody, sc-52. However, we offer several different options. Please contact our Technical Service Department for a recommendation based on your intended research application(s) and species reactivity.
Respondida por: Technical Support
Fecha de publicación: 2019-05-25

I used immunofluorescence staining in the hypothalamus of rat, and as a result c-fos was expressed in the cytoplasm. It's not expressed in the nucleus. Is it correct? 0 answers

Preguntado por: Emma Li
Thank you for your question. According to the Human Protein Atlas, c-Fos (FOS) is localized to the nucleoplasm. Nuclear and cytoplasmic expression may be detected in most tissues. https://www.proteinatlas.org/ENSG00000170345-FOS/pathology
Respondida por: Technical Support
Fecha de publicación: 2019-05-25

Can this antibody be used for the detection of c-fos in the macaque monkey. If not, which of your antibodies would you recommend?

Preguntado por: myprefrontal
Thank you for your question. We have not tested this antibody for reactivity with macaque, and are not aware of any publications citing use of the antibody in that species. We do offer an alternative antibody, c-Fos (6-2H-2F): sc-447, that has been published at least twice for detection of c-Fos in monkey. Please see PMID's: 9108029 and 11937718 for those references. A link to that product page on our website is below. https://www.scbt.com/scbt/product/c-fos-antibody-6-2h-2f
Respondida por: Technical Support
Fecha de publicación: 2017-11-01

Can I still buy monoclonal antibodies from this company?

Preguntado por: KarenLindquist
Thank you for your question! Yes, you can certainly buy monoclonal antibodies from us! Please contact us at any time and we'd be glad to help you! You can reach us by phone at 800-457-3801 or by email at [email protected] or by live chat directly on our website, www.scbt.com.
Respondida por: Tech Service
Fecha de publicación: 2017-05-29

Could you please advise if this antibody is able to detect Fra proteins as is the case with the discontinued sc 253.

Preguntado por: Conor
Thank you for your question. The equivalent replacement mouse monoclonal antibodies to sc-253 are sc-166940: c-Fos (E-8) and sc-271243: c-Fos (C-10). Both of these mouse monoclonal antibodies are raised against the same peptide antigen used to generate the polyclonal antibody, sc-253. These recommended replacement mouse monoclonal antibodies are also recommended for detection of c-Fos, Fos B, Fra-1 and Fra-2 of mouse, rat, human, zebrafish and avian origin. Links to these products are provided below for your reference. https://www.scbt.com/scbt/product/c-fos-antibody-e-8 https://www.scbt.com/scbt/product/c-fos-antibody-c-10
Respondida por: Technical Support
Fecha de publicación: 2017-01-19

I want to detect c-fos in my porcine samples. Will this antibody work?

Preguntado por: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Respondida por: TechService7
Fecha de publicación: 2016-12-23
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Rated 5 de 5 por de - We examined the effects of a 3-week treatment of olanzapine (Olz) on c-Fos protein expression in stress-relevant brain sub/ regions, its relationship with isolation-induced behavioral changes, and potential sites of Olz action on control and male rats exposed to 6 weeks of chronic social isolation an animal model of depression.
Fecha de publicación: 2018-12-07
Rated 5 de 5 por de 功能很齐全也很棒 我两个月前购买了这个产品,用它来做免疫荧光看的非常清楚,我想再买一盒接着做,同时我最满意的是它的性价比,很实惠也很实用,值得购买
Fecha de publicación: 2018-05-21
Rated 2 de 5 por de It does not work well It did not work as well as other company's ab when I tested it by 1: 500.
Fecha de publicación: 2018-04-03
Rated 2 de 5 por de No nuclear staining Level of expression appears comparable to other fos antibodies, but does not stain the the nucleus and non-specific binding is present at high concentrations.
Fecha de publicación: 2018-03-14
Rated 1 de 5 por de Not satisfied! I tried a free sample of this antibody on free floating mouse brain sections and it didn't work. I used a concentration of 1:50 for the primary antibody, and 1:300 for secondary antibody (anti-mouse 488) and I didn't got any signal. Really not satisfied.
Fecha de publicación: 2018-02-23
Rated 5 de 5 por de 功能很出色 我上一个月染得的fos,很出色,背景很清晰,很清晰。我上一个月染得的fos,很出色,背景很清晰,很清晰。
Fecha de publicación: 2018-02-05
Rated 1 de 5 por de Disappointed I tried a sample of this antibody on my free-floating mouse brain sections which I have another set stained with my old, running out antibody to compare. It did not work at all at concentration ranging 1:100-2000.
Fecha de publicación: 2017-11-27
Rated 1 de 5 por de Did not label cFos cells in sheep hypothalamus Using free-floating sections from sheep tissue, and an immunoperoxidase method of IHC, we did not see any immunostained cells using this antibody at dilutions of 1: 500, 1:1000, 1:2000.
Fecha de publicación: 2017-11-08
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