MBP-probe Antibody (P2F1): sc-32747

MBP-probe Antibody (P2F1) is a mouse monoclonal IgG2a antibody that detects maltose binding protein (MBP) by western blotting (WB) and immunoprecipitation (IP). MBP is a 370 amino acid protein derived from the E. coli malE gene, playing a crucial role in the transport and binding of maltose and maltodextrins, which are essential for bacterial metabolism. The ability of MBP to bind maltose with high affinity not only facilitates the uptake of these sugars but also serves as a valuable tool in molecular biology for the purification of recombinant proteins. By utilizing MBP as a fusion tag, researchers can easily isolate target proteins through affinity chromatography, leveraging the specific interaction between MBP and amylose resin. This method allows for the efficient purification of proteins, which is vital for various applications in research and biotechnology. Anti-MBP antibody (P2F1) is specifically designed to recognize and bind to the MBP tag, enabling the visualization and analysis of MBP-fusion proteins in experimental setups.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MBP-probe Antibody (P2F1) References:

1. Maltose-binding protein is open in the catalytic transition state for ATP hydrolysis during maltose transport.  |  Austermuhle, MI., et al. 2004. J Biol Chem. 279: 28243-50. PMID: 15117946
2. A Maltose-Binding Protein Fusion Construct Yields a Robust Crystallography Platform for MCL1.  |  Clifton, MC., et al. 2015. PLoS One. 10: e0125010. PMID: 25909780
3. Easy mammalian expression and crystallography of maltose-binding protein-fused human proteins.  |  Bokhove, M., et al. 2016. J Struct Biol. 194: 1-7. PMID: 26850170
4. Open and Closed Form of Maltose Binding Protein in Its Native and Molten Globule State As Studied by Electron Paramagnetic Resonance Spectroscopy.  |  Selmke, B., et al. 2018. Biochemistry. 57: 5507-5512. PMID: 30004675
5. Evaluation of Maltose Binding Protein-Tagged hATR Kinase Domain Catalytic Activity with p53 Ser-15 Phosphorylation.  |  Bhakuni, R., et al. 2018. Biochemistry. 57: 6592-6603. PMID: 30380295
6. Both Ligands and Macromolecular Crowders Preferentially Bind to Closed Conformations of Maltose Binding Protein.  |  Ghosh, A., et al. 2019. Biochemistry. 58: 2208-2217. PMID: 30950267
7. Passenger sequences can promote interlaced dimers in a common variant of the maltose-binding protein.  |  Momin, AA., et al. 2019. Sci Rep. 9: 20396. PMID: 31892719
8. Single chain variable fragment fused to maltose binding protein: a modular nanocarrier platform for the targeted delivery of antitumorals.  |  Reche-Perez, FJ., et al. 2021. Biomater Sci. 9: 1728-1738. PMID: 33432316
9. Characterization of maltose-binding protein-fused heparinases with enhanced thermostability by application of rigid and flexible linkers.  |  Wu, X., et al. 2025. Biotechnol Appl Biochem. 72: 5-16. PMID: 39072851
10. Maltose binding protein (MBP) fusion proteins with low or no affinity to amylose resins can be single-step purified using a novel anti-MBP monoclonal antibody.  |  Park, JH., et al. 1998. Mol Cells. 8: 709-16. PMID: 9895124

The preferred MBP-probe antibody is MBP-probe Antibody (R29.6): sc-13564.