Integrin αM/CD11b Double Nickase Plasmid (h): sc-400563-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin αM/CD11b Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Integrin αM/CD11b Double Nickase Plasmid (h) and Integrin αM/CD11b Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ITGAM. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin αM/CD11b Antibody (2LPM19c): sc-20050
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin αM/CD11b Double Nickase Plasmid (h) | sc-400563-NIC | 20 µg | $410.00 | |||
Integrin αM/CD11b Double Nickase Plasmid (h2) | sc-400563-NIC-2 | 20 µg | $410.00 |
Human ITGAM encodes integrin αM (CD11b), which heterodimerizes with ITGB2 to form Mac-1/CR3, a leukocyte adhesion and complement receptor central to innate immune function. CD11b mediates firm adhesion and transendothelial migration through ICAM interactions, supports phagocytosis of iC3b-opsonized targets, and contributes to outside-in signaling that shapes actin remodeling and inflammatory gene programs. These activities interface with integrin signaling, Fc receptor cross-talk, and chemokine-guided trafficking in myeloid cells such as neutrophils, monocytes, macrophages, and select dendritic cell subsets. Dysregulated CD11b-dependent adhesion and effector signaling are frequently examined in inflammation and autoimmunity, infectious disease pathogenesis, and myeloid cell behavior in tumor-associated immune microenvironments.
Integrin αM/CD11b Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ITGAM locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ITGAM. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ITGAM function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ITGAM-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.