Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Integrin α1/ITGA1/CD49a CRISPR/Cas9 KO Plasmid (h): sc-401275
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Integrin α1/ITGA1/CD49a CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Integrin α1/ITGA1/CD49a genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Integrin α1/ITGA1/CD49a Antibody (A-9): sc-271034
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Integrin α1/ITGA1/CD49a CRISPR/Cas9 KO Plasmid (h) | sc-401275 | 20 µg | $397.00 |
Overview
ITGA1 encodes integrin α1 (CD49a), which heterodimerizes with integrin β1 to form a collagen- and laminin-binding receptor that mediates cell–extracellular matrix adhesion and mechanotransduction. Through focal adhesion assembly and signaling via FAK/Src, PI3K–AKT, and MAPK pathways, integrin α1 influences cytoskeletal organization, migration, proliferation, and survival in a context-dependent manner. ITGA1 activity contributes to regulation of tissue remodeling and inflammatory cell positioning by shaping adhesion dynamics and downstream transcriptional responses. Altered integrin α1/β1 signaling has been linked to dysregulated cell–matrix interactions observed in fibrosis, tumor–stroma crosstalk, and immune microenvironment remodeling, making it a useful target for pathway-focused studies.
Integrin α1/ITGA1/CD49a CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ITGA1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ITGA1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ITGA1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Integrin α1/ITGA1/CD49a protein expression.
This CRISPR knockout system enables efficient generation of ITGA1-deficient cell models for investigation of Integrin α1/ITGA1/CD49a signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ITGA1 exon(s) critical for Integrin α1/ITGA1/CD49a function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ITGA1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Integrin α1/ITGA1/CD49a CRISPR/Cas9 KO Plasmid (h) and Integrin α1/ITGA1/CD49a CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ITGA1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Integrin α1/ITGA1/CD49a HDR Plasmid (h) and Integrin α1/ITGA1/CD49a HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ITGA1 homology arms to support homology-directed repair at defined ITGA1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.