HSP 75 Antibody (F-7): sc-390061
- HSP 75 Antibody (F-7) is a mouse monoclonal IgG1 κ HSP 75 antibody provided at 200 µg/ml
- raised against amino acids 522-644 mapping near the C-terminus of HSP 75 of human origin
- HSP 75 Antibody (F-7) is recommended for detection of HSP 75 of human origin by WB, IP, IF and ELISA
- Anti-HSP 75 Antibody (F-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for HSP 75 Antibody (F-7) for WB applications. This reagent is now offered in a bundle with HSP 75 Antibody (F-7) (see ordering information below).
HSP 75 Antibody (F-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects HSP 75 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HSP 75 (F-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The heat shock protein 75, also known as tumor necrosis factor type 1 receptor-associated protein (TRAP-1), is a crucial mitochondrial chaperone composed of 704 amino acids and belongs to the heat shock protein 90 family. HSP 75 assists in proper folding and assembly of proteins within mitochondria, which is vital for maintaining mitochondrial function and cellular homeostasis. HSP 75 plays a significant role in protecting cells from stress-induced damage, particularly under conditions of oxidative stress, by preventing aggregation of misfolded proteins and facilitating their refolding or degradation. This protective mechanism is essential for cell survival and function, especially in tissues with high metabolic demands such as skeletal muscle, liver, heart, brain, pancreas, lung, and placenta, where HSP 75 is abundantly expressed.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HSP 75 Antibody (F-7) References:
- Translational control in the stress adaptive response of cancer cells: a novel role for the heat shock protein TRAP1. | Matassa, DS., et al. 2013. Cell Death Dis. 4: e851. PMID: 24113185
- Aberrantly upregulated TRAP1 is required for tumorigenesis of breast cancer. | Zhang, B., et al. 2015. Oncotarget. 6: 44495-508. PMID: 26517089
- Stress-Adaptive Response in Ovarian Cancer Drug Resistance: Role of TRAP1 in Oxidative Metabolism-Driven Inflammation. | Amoroso, MR., et al. 2017. Adv Protein Chem Struct Biol. 108: 163-198. PMID: 28427560
- Mitochondrial chaperone, TRAP1 as a potential pharmacological target to combat cancer metabolism. | Ramkumar, B., et al. 2020. Mitochondrion. 50: 42-50. PMID: 31669620
- HSPA1A, HSPA1L and TRAP1 heat shock genes may be associated with prognosis in ovarian epithelial cancer. | De Andrade, WP., et al. 2020. Oncol Lett. 19: 359-367. PMID: 31897148
- The mitochondrial HSP90 paralog TRAP1 forms an OXPHOS-regulated tetramer and is involved in mitochondrial metabolic homeostasis. | Joshi, A., et al. 2020. BMC Biol. 18: 10. PMID: 31987035
- Suppressing TRAP1 sensitizes glioblastoma multiforme cells to temozolomide. | Wang, N., et al. 2021. Exp Ther Med. 22: 1246. PMID: 34539842
- O-GlcNAcylation suppresses TRAP1 activity and promotes mitochondrial respiration. | Kim, S., et al. 2022. Cell Stress Chaperones. 27: 573-585. PMID: 35976490
- Inhibition of TRAP1 Accelerates the DNA Damage Response, Activation of the Heat Shock Response and Metabolic Reprogramming in Colon Cancer Cells. | Bhasin, N., et al. 2023. Front Biosci (Landmark Ed). 28: 227. PMID: 37796715
- The differential roles of heat shock protein 90 isoforms in skin inflammation: Anti-inflammatory potential of TRAP1 inhibition. | Ben Abdallah, H., et al. 2025. J Invest Dermatol.. PMID: 39978584
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSP 75 Antibody (F-7) | sc-390061 | 200 µg/ml | $322.00 | |||
HSP 75 Antibody (F-7): m-IgG Fc BP-HRP Bundle | sc-526118 | 200 µg Ab; 10 µg BP | $361.00 | |||
HSP 75 Antibody (F-7) AC | sc-390061 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HSP 75 Antibody (F-7) HRP | sc-390061 HRP | 200 µg/ml | $322.00 | |||
HSP 75 Antibody (F-7) FITC | sc-390061 FITC | 200 µg/ml | $336.00 | |||
HSP 75 Antibody (F-7) PE | sc-390061 PE | 200 µg/ml | $349.00 | |||
HSP 75 Antibody (F-7) Alexa Fluor® 488 | sc-390061 AF488 | 200 µg/ml | $364.00 | |||
HSP 75 Antibody (F-7) Alexa Fluor® 546 | sc-390061 AF546 | 200 µg/ml | $364.00 | |||
HSP 75 Antibody (F-7) Alexa Fluor® 594 | sc-390061 AF594 | 200 µg/ml | $364.00 | |||
HSP 75 Antibody (F-7) Alexa Fluor® 647 | sc-390061 AF647 | 200 µg/ml | $364.00 | |||
HSP 75 Antibody (F-7) Alexa Fluor® 680 | sc-390061 AF680 | 200 µg/ml | $364.00 | |||
HSP 75 Antibody (F-7) Alexa Fluor® 790 | sc-390061 AF790 | 200 µg/ml | $364.00 |