HLA-DQB1 Double Nickase Plasmid (h): sc-403051-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HLA-DQB1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HLA-DQB1 Double Nickase Plasmid (h) and HLA-DQB1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HLA-DQB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HLA-DQB1 Antibody (IIB3): sc-59247
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HLA-DQB1 Double Nickase Plasmid (h) | sc-403051-NIC | 20 µg | $410.00 | |||
HLA-DQB1 Double Nickase Plasmid (h2) | sc-403051-NIC-2 | 20 µg | $410.00 |
HLA-DQB1 encodes the β chain of the HLA-DQ heterodimeric MHC class II receptor expressed primarily on professional antigen-presenting cells, where it binds and presents extracellular peptide antigens to CD4+ T cells. Through peptide loading in the endosomal/lysosomal compartment and surface presentation, HLA-DQB1 contributes to adaptive immune activation, peripheral tolerance, and cytokine-driven immune signaling. Allelic variation and altered expression of HLA-DQB1 influence antigen repertoire and T cell reactivity, linking this locus to immune-mediated disease susceptibility and transplant-related immune recognition. As part of the HLA class II region, HLA-DQB1 is widely studied in immunogenetics, antigen presentation dynamics, and mechanisms of autoimmunity and inflammation.
HLA-DQB1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HLA-DQB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HLA-DQB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HLA-DQB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HLA-DQB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.