HDAC5 Anticuerpo (B-11) recomendado para detectarHDAC5 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA

HDAC5 Anticuerpo (B-11): sc-133106

HDAC5 Anticuerpo (B-11) clasificado 5.0 de 5 por 7.
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Ficha técnica

    • HDAC5 Anticuerpo (B-11) es un monoclonal de ratón IgG2b (kappa light chain) proporcionado como 200 µg/ml
    • producido contra los amino ácidos 371-443 de HDAC5 de origen human
    • HDAC5 Anticuerpo (B-11) es recomendado para detectar HDAC5 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA
    • HDAC5 Anticuerpo (B-11) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de HDAC5 (B-11): sc-133106.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for HDAC5 Antibody (B-11) for WB and IHC(P) applications. This reagent is now offered in a bundle with HDAC5 Antibody (B-11) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Can this antibody be used on fixed mouse tissue in IF to detect HDAC5 via a secondary? Will there be too much background without a mouse on mouse block?

Preguntado por: pat68
Thank you for inquiring with us. This antibody can be used for detection of HDAC5 in mouse samples as long as the tissue isn't known to have high endogenous IgG levels. We recommend using our Mouse IgG Binding Proteins as secondaries (https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-130b1ry) to reduce non-specific staining. Mouse-on-mouse kits can also be used to reduce potential non-specific staining. Alternatively try our HDAC5 (C-11) antibody sc-133225 which is available conjugated to AF488, AF647, FITC, and PE.
Respondida por: Technical Service
Fecha de publicación: 2017-04-19

Can you recommend a positive control to use with HDAC5 (B-11): sc-133106 monoclonal antibody for Western blot?

Preguntado por: jenni
We recommend IMR-32 (sc-2409) or KNRK (sc-2214) whole cell lysates to use as positive controls as we have Western blot data showing specific detection of HDAC5 in these cell lines.
Respondida por: Technical Support 4
Fecha de publicación: 2017-01-17
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Rated 5 de 5 por de band was nice We used it in our WB and it worked well, HDAC5 gave best signal to background
Fecha de publicación: 2017-04-30
Rated 5 de 5 por de Great Antibody for WB, IHC(P), and IF HDAC5 (B-11) works great for WB, IHC(P), and IF. Nice clean band in positive control KNRK Whole Cell Lysate: sc-2214 at 140-150 kDa.
Fecha de publicación: 2017-01-13
Rated 5 de 5 por de One of the great antibody from SCBT One of the great antibody from SCBT. completely validated in HDAC5 knockout mice. Used in Western Blot.
Fecha de publicación: 2016-04-27
Rated 5 de 5 por de Used for both WB and IP in human cells Used for both WB and IP in human cells- got correct band at 1:500 dilution.
Fecha de publicación: 2015-10-28
Rated 5 de 5 por de the antibody is very good and the bands the antibody is very good and the bands are clear .
Fecha de publicación: 2015-04-14
Rated 5 de 5 por de Positive western blot results in IMR Positive western blot results in IMR-32 and KNRK whole cell lysates.
Fecha de publicación: 2015-03-17
Rated 5 de 5 por de Cytoplasmic localization detected in immunofluorescence Cytoplasmic localization detected in immunofluorescence staining of formalin-fixed HepG2 cells. -SCBT QC
Fecha de publicación: 2015-03-08
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