HDAC3 Anticuerpo (B-12) es un monoclonal de ratón IgG2a κ, ver las 25 publicaciones, proporcionado como 200 µg/ml
Producido contra los amino ácidos 330-428 en el C-terminus de HDAC3 de origen human
HDAC3 Anticuerpo (B-12) es recomendado para detectar HDAC3 de mouse, rat y human origen, mediante WB, IP, IF y ELISA; también reactivo con otras especies, incluyendo anyd equine, canine, bovine and porcine
HDAC3 Anticuerpo (B-12) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
Contacte con nosotros para recibir GRATIS 10 µg de muestra de HDAC3 (B-12): sc-17795.
HDAC para análisis funcionales de las repuestas celulares a HDAC
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In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional factor Rpd3p, have been identified as histone deacetylases.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended secondary reagent to be used with this mouse monoclonal primary?
Preguntado por: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here:
Rated 5 de
Very popular HDAC3 clone!This mouse monoclonal, HDAC3 Antibody (B-12 is a very popular choice and has been featured in 15 publications for a variety of research applications including WB, IP and ChIP.
Fecha de publicación: 2017-01-26
Rated 3 de
Basudev Chowdhury de
WB correct mw sized band~49 KDa band observed by WB on 10 ug MDA-MB-231 whole cell lysates at 1:1000 dilution. 3 more unidentifiable bands visible.
Fecha de publicación: 2016-12-12
Rated 4 de
This has worked very well in WB&IP with RAW264This has worked very well in WB&IP with RAW264.7 cells.
Fecha de publicación: 2015-11-19
Rated 5 de
Publishable data in coimmunoprecipitationPublishable data in coimmunoprecipitation and Western blotting with this antibody -SCBT Publication Review
Fecha de publicación: 2015-02-10
Rated 5 de
This antibody works in many different humanThis antibody works in many different human cell lines such as MCF10A, MCF7, A431 and Raji. -SCBT Publication Review
Fecha de publicación: 2015-01-08
Rated 4 de
Good specificity and signal strength withGood specificity and signal strength with some background bands -SCBT QC
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