GPR3 Antibody (B-5): sc-390276

GPR3 Antibody (B-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects GPR3 protein of mouse, rat, and, to a lesser extent, human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GPR3 Antibody (B-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. G protein-coupled receptor 3 (GPR3), also known as the ACCA orphan receptor, is a 330 amino acid protein that belongs to the G protein-coupled receptor 1 family. The structure of GPR3 is significant as a multi-pass membrane protein, which spans the cellular membrane multiple times, facilitating signal transduction. This structural characteristic enables interaction with G proteins, which activate adenylate cyclase, leading to cyclic adenosine monophosphate (cAMP) production. GPR3 is predominantly expressed in the central nervous system, where GPR3 plays a vital role in stimulating cAMP production, thereby influencing neurite outgrowth and myelin inhibition. Additionally, in oocytes, GPR3′s ability to regulate cAMP levels is essential for controlling meiosis and preventing premature progesterone-induced meiotic maturation. Mice lacking GPR3 can reproduce; however, these mice exhibit a lack of control over oocyte maturation, resulting in nondeveloping early embryos and fragmented oocytes as they age.

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GPR3 Antibody (B-5) References:

1. The G-protein-coupled receptors GPR3 and GPR12 are involved in cAMP signaling and maintenance of meiotic arrest in rodent oocytes.  |  Hinckley, M., et al. 2005. Dev Biol. 287: 249-61. PMID: 16229830
2. [Oocyte-cumulus dialog].  |  Feuerstein, P., et al. 2006. Gynecol Obstet Fertil. 34: 793-800. PMID: 16959524
3. Regulation of meiotic maturation.  |  Richard, FJ. 2007. J Anim Sci. 85: E4-6. PMID: 17040950
4. Neural expression of G protein-coupled receptors GPR3, GPR6, and GPR12 up-regulates cyclic AMP levels and promotes neurite outgrowth.  |  Tanaka, S., et al. 2007. J Biol Chem. 282: 10506-15. PMID: 17284443
5. Molecular cloning of an orphan G-protein-coupled receptor that constitutively activates adenylate cyclase.  |  Eggerickx, D., et al. 1995. Biochem J. 309 (Pt 3): 837-43. PMID: 7639700
6. Isolation and chromosomal localization of a novel human G-protein-coupled receptor (GPR3) expressed predominantly in the central nervous system.  |  Iismaa, TP., et al. 1994. Genomics. 24: 391-4. PMID: 7698767
7. Cloning of human genes encoding novel G protein-coupled receptors.  |  Marchese, A., et al. 1994. Genomics. 23: 609-18. PMID: 7851889
8. Molecular cloning and chromosomal localization of human genes encoding three closely related G protein-coupled receptors.  |  Song, ZH., et al. 1995. Genomics. 28: 347-9. PMID: 8530049

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GPR3 Antibody (B-5) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-GPR3 Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GPR3 Antibody (B-5) Specifications

Alternate Product Name: GPR3

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-50

Epitope Target: GPR3

Epitope Species: mouse

Product Reactivity/Detection: GPR3

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for GPR3 Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin