GlyT2 Antibody (B-4): sc-390090

GlyT2 Antibody (B-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects GlyT2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). GlyT2 (B-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GlyT2, a member of the Na+/Cl- dependent neurotransmitter transporter superfamily, plays a critical role in terminating neurotransmission at glycinergic synapses, which is essential for maintaining the balance of excitatory and inhibitory signals in the central nervous system. GlyT2 is primarily localized in the gray matter of the brain and spinal cord, as well as in peripheral tissues such as macrophages and mast cells, highlighting GlyT2′s importance in both central and peripheral signaling pathways. The unique expression pattern of GlyT2, in contrast to GlyT1, which is restricted to the white matter of the central nervous system, suggests that glycine may function not only as a neurotransmitter but also as a chemical messenger outside the central nervous system, potentially influencing various physiological processes. Understanding the distribution and function of GlyT2 is vital for elucidating GlyT2′s role in neurophysiology and implications in neurological disorders.

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GlyT2 Antibody (B-4) References:

1. Cloning, functional characterisation and population analysis of a variant form of the human glycine type 2 transporter.  |  Evans, J., et al. 1999. FEBS Lett. 463: 301-6. PMID: 10606742
2. Surface-localized glycine transporters 1 and 2 function as monomeric proteins in Xenopus oocytes.  |  Horiuchi, M., et al. 2001. Proc Natl Acad Sci U S A. 98: 1448-53. PMID: 11171971
3. Cloning and expression of a glycine transporter from mouse brain.  |  Liu, QR., et al. 1992. FEBS Lett. 305: 110-4. PMID: 1618338
4. Calcium-Dependent Regulation of the Neuronal Glycine Transporter GlyT2 by M2 Muscarinic Acetylcholine Receptors.  |  Jiménez, E., et al. 2022. Neurochem Res. 47: 190-203. PMID: 33765249
5. Molecular and Electrophysiological Characterization of Dorsal Horn Neurons in a GlyT2-iCre-tdTomato Mouse Line.  |  He, X., et al. 2021. J Pain Res. 14: 907-921. PMID: 33854367
6. The presynaptic glycine transporter GlyT2 is regulated by the Hedgehog pathway in vitro and in vivo.  |  de la Rocha-Muñoz, A., et al. 2021. Commun Biol. 4: 1197. PMID: 34663888
7. Synergistic Control of Transmitter Turnover at Glycinergic Synapses by GlyT1, GlyT2, and Asc-1.  |  Eulenburg, V. and Hülsmann, S. 2022. Int J Mol Sci. 23: PMID: 35269698
8. Bidirectional Modulation of Nociception by GlyT2+ Neurons in the Ventrolateral Periaqueductal Gray.  |  Assareh, N., et al. 2023. eNeuro. 10: PMID: 37253591
9. Gene structure and glial expression of the glycine transporter GlyT1 in embryonic and adult rodents.  |  Adams, RH., et al. 1995. J Neurosci. 15: 2524-32. PMID: 7891186
10. Cloning of the human glycine transporter type 1: molecular and pharmacological characterization of novel isoform variants and chromosomal localization of the gene in the human and mouse genomes.  |  Kim, KM., et al. 1994. Mol Pharmacol. 45: 608-17. PMID: 8183239
11. Two glycine transporter variants with distinct localization in the CNS and peripheral tissues are encoded by a common gene.  |  Borowsky, B., et al. 1993. Neuron. 10: 851-63. PMID: 8494645
12. The glycine transporter GLYT2 is a reliable marker for glycine-immunoreactive neurons.  |  Poyatos, I., et al. 1997. Brain Res Mol Brain Res. 49: 63-70. PMID: 9387864

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GlyT2 Antibody (B-4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-GlyT2 Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GlyT2 Antibody (B-4) Specifications

Alternate Product Name: GlyT2

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 50-204

Epitope Target: GlyT2

Epitope Species: human

Product Reactivity/Detection: GlyT2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for GlyT2 Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin